Detailed information of TA system TA067708

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2213388..2214091
• Replicon: chromosome
• Accession: NZ_CP017597
• Organism: Mycobacterium tuberculosis strain Beijing-like/50148

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: BJM03_RS10610
• Protein ID: WP_003409778.1
• Coordinates: 2213388..2213717 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: BJM03_RS10615
• Protein ID: WP_003409780.1
• Coordinates: 2213714..2214091 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJM03_RS10590	2209771..2210841	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
BJM03_RS10595	2210838..2211353	+	516	WP_003409718.1	flavin reductase family protein	-
BJM03_RS10600	2211350..2212411	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
BJM03_RS10605	2212408..2213178	+	771	WP_003409775.1	SDR family oxidoreductase	-
BJM03_RS10610	2213388..2213717	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BJM03_RS10615	2213714..2214091	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
BJM03_RS10620	2214088..2214678	-	591	WP_003409784.1	SEC-C domain-containing protein	-
BJM03_RS10625	2214733..2216097	+	1365	WP_003910878.1	HNH endonuclease	-
BJM03_RS10630	2216252..2216704	-	453	WP_003899095.1	lipoprotein	-
BJM03_RS10635	2216768..2217169	+	402	WP_003409869.1	hypothetical protein	-
BJM03_RS10640	2217162..2217344	-	183	WP_003409870.1	hypothetical protein	-
BJM03_RS10645	2217458..2217808	-	351	WP_003409871.1	hypothetical protein	-
BJM03_RS10650	2217819..2218721	-	903	WP_003899097.1	hypothetical protein	-
BJM03_RS10655	2218742..2218933	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T67708 WP_003409778.1 NZ_CP017597:c2213717-2213388 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T67708 NZ_CP017597:c2213717-2213388 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT67708 WP_003409780.1 NZ_CP017597:c2214091-2213714 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT67708 NZ_CP017597:c2214091-2213714 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8