Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1261572..1262203 Replicon chromosome
Accession NZ_CP017597
Organism Mycobacterium tuberculosis strain Beijing-like/50148

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag BJM03_RS06185 Protein ID WP_003405820.1
Coordinates 1261572..1261883 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag BJM03_RS06190 Protein ID WP_003405836.1
Coordinates 1261883..1262203 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJM03_RS06165 1257053..1258477 - 1425 WP_003405805.1 class II fumarate hydratase -
BJM03_RS06170 1258508..1259596 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
BJM03_RS06175 1259652..1260296 + 645 WP_003915494.1 DUF4245 domain-containing protein -
BJM03_RS06180 1260303..1261460 - 1158 WP_003898727.1 AI-2E family transporter -
BJM03_RS06185 1261572..1261883 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
BJM03_RS06190 1261883..1262203 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
BJM03_RS06195 1262213..1263744 + 1532 Protein_1174 carboxylesterase/lipase family protein -
BJM03_RS06200 1263751..1264863 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
BJM03_RS06205 1264873..1265130 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
BJM03_RS06210 1265120..1266367 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
BJM03_RS06215 1266364..1267002 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T67698 WP_003405820.1 NZ_CP017597:c1261883-1261572 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T67698 NZ_CP017597:c1261883-1261572 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT67698 WP_003405836.1 NZ_CP017597:c1262203-1261883 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT67698 NZ_CP017597:c1262203-1261883 [Mycobacterium tuberculosis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References