Detailed information of TA system TA067680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 394852..395423
• Replicon: chromosome
• Accession: NZ_CP017597
• Organism: Mycobacterium tuberculosis strain Beijing-like/50148

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: BJM03_RS01760
• Protein ID: WP_003401560.1
• Coordinates: 394852..395154 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: BJM03_RS01765
• Protein ID: WP_003401563.1
• Coordinates: 395202..395423 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJM03_RS01740	390330..391133	-	804	WP_003401540.1	hypothetical protein	-
BJM03_RS01745	391143..392540	-	1398	WP_003401544.1	sulfatase	-
BJM03_RS01750	392719..394485	+	1767	WP_031648214.1	PE family protein	-
BJM03_RS01755	394628..394855	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
BJM03_RS01760	394852..395154	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
BJM03_RS01765	395202..395423	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
BJM03_RS01770	395420..395845	+	426	WP_003401566.1	PIN domain nuclease	-
BJM03_RS01775	395981..396613	+	633	WP_003905275.1	TetR/AcrR family transcriptional regulator	-
BJM03_RS01780	396610..397518	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T67680 WP_003401560.1 NZ_CP017597:394852-395154 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T67680 NZ_CP017597:394852-395154 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT67680 WP_003401563.1 NZ_CP017597:395202-395423 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT67680 NZ_CP017597:395202-395423 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CG48