Detailed information of TA system TA067648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2180286..2180989
• Replicon: chromosome
• Accession: NZ_CP017596
• Organism: Mycobacterium tuberculosis strain Beijing/391

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: BJM02_RS10455
• Protein ID: WP_003409778.1
• Coordinates: 2180286..2180615 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: BJM02_RS10460
• Protein ID: WP_003409780.1
• Coordinates: 2180612..2180989 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJM02_RS10435	2176669..2177739	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
BJM02_RS10440	2177736..2178251	+	516	WP_003409718.1	flavin reductase family protein	-
BJM02_RS10445	2178248..2179309	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
BJM02_RS10450	2179306..2180076	+	771	WP_003409775.1	SDR family oxidoreductase	-
BJM02_RS10455	2180286..2180615	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BJM02_RS10460	2180612..2180989	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
BJM02_RS10465	2180986..2181576	-	591	WP_003409784.1	SEC-C domain-containing protein	-
BJM02_RS10470	2181631..2182995	+	1365	WP_003903691.1	HNH endonuclease	-
BJM02_RS10475	2183150..2183602	-	453	WP_003899095.1	lipoprotein	-
BJM02_RS10480	2183666..2184067	+	402	WP_003409869.1	hypothetical protein	-
BJM02_RS10485	2184060..2184242	-	183	WP_003409870.1	hypothetical protein	-
BJM02_RS10490	2184356..2184706	-	351	WP_003409871.1	hypothetical protein	-
BJM02_RS10495	2184717..2185619	-	903	WP_003899097.1	hypothetical protein	-
BJM02_RS10500	2185640..2185831	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T67648 WP_003409778.1 NZ_CP017596:c2180615-2180286 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T67648 NZ_CP017596:c2180615-2180286 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT67648 WP_003409780.1 NZ_CP017596:c2180989-2180612 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT67648 NZ_CP017596:c2180989-2180612 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8