Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2880392..2881023 Replicon chromosome
Accession NZ_CP017595
Organism Mycobacterium tuberculosis strain Beijing-like/38774

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag BJM01_RS14050 Protein ID WP_003413174.1
Coordinates 2880646..2881023 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag BJM01_RS14045 Protein ID WP_003413167.1
Coordinates 2880392..2880649 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJM01_RS14010 2876306..2876620 + 315 WP_009937839.1 hypothetical protein -
BJM01_RS14015 2876916..2878127 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
BJM01_RS14025 2878254..2878913 + 660 WP_003902296.1 hypothetical protein -
BJM01_RS14030 2878910..2879573 + 664 Protein_2646 hypothetical protein -
BJM01_RS21710 2879570..2879847 + 278 Protein_2647 type II toxin-antitoxin system VapB family antitoxin -
BJM01_RS14040 2879940..2880353 + 414 WP_003413164.1 PIN domain nuclease -
BJM01_RS14045 2880392..2880649 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
BJM01_RS14050 2880646..2881023 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
BJM01_RS14055 2881039..2881413 - 375 WP_003413177.1 hypothetical protein -
BJM01_RS14060 2881513..2881908 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
BJM01_RS14065 2881905..2882150 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
BJM01_RS14075 2882561..2882980 - 420 WP_003413190.1 A24 family peptidase -
BJM01_RS14080 2882992..2883801 - 810 WP_003413193.1 shikimate dehydrogenase -
BJM01_RS14085 2883798..2885051 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
BJM01_RS14090 2885044..2885556 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T67602 WP_003413174.1 NZ_CP017595:2880646-2881023 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T67602 NZ_CP017595:2880646-2881023 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT67602 WP_003413167.1 NZ_CP017595:2880392-2880649 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT67602 NZ_CP017595:2880392-2880649 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References