Detailed information of TA system TA067588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2186141..2186844
• Replicon: chromosome
• Accession: NZ_CP017595
• Organism: Mycobacterium tuberculosis strain Beijing-like/38774

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: BJM01_RS10485
• Protein ID: WP_003409778.1
• Coordinates: 2186141..2186470 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: BJM01_RS10490
• Protein ID: WP_003409780.1
• Coordinates: 2186467..2186844 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJM01_RS10465	2182524..2183594	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
BJM01_RS10470	2183591..2184106	+	516	WP_003409718.1	flavin reductase family protein	-
BJM01_RS10475	2184103..2185164	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
BJM01_RS10480	2185161..2185931	+	771	WP_003409775.1	SDR family oxidoreductase	-
BJM01_RS10485	2186141..2186470	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BJM01_RS10490	2186467..2186844	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
BJM01_RS10495	2186841..2187431	-	591	WP_003409784.1	SEC-C domain-containing protein	-
BJM01_RS10500	2187486..2188850	+	1365	WP_003903691.1	HNH endonuclease	-
BJM01_RS10505	2189005..2189457	-	453	WP_003899095.1	lipoprotein	-
BJM01_RS10510	2189521..2189922	+	402	WP_003409869.1	hypothetical protein	-
BJM01_RS10515	2189915..2190097	-	183	WP_003409870.1	hypothetical protein	-
BJM01_RS10520	2190211..2190561	-	351	WP_003409871.1	hypothetical protein	-
BJM01_RS10525	2190572..2191474	-	903	WP_003899097.1	hypothetical protein	-
BJM01_RS10530	2191495..2191686	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T67588 WP_003409778.1 NZ_CP017595:c2186470-2186141 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T67588 NZ_CP017595:c2186470-2186141 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT67588 WP_003409780.1 NZ_CP017595:c2186844-2186467 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT67588 NZ_CP017595:c2186844-2186467 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8