Detailed information of TA system TA067542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2891091..2891722
• Replicon: chromosome
• Accession: NZ_CP017594
• Organism: Mycobacterium tuberculosis strain Beijing-like/36918

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: BJM00_RS14060
• Protein ID: WP_003413174.1
• Coordinates: 2891345..2891722 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: BJM00_RS14055
• Protein ID: WP_003413167.1
• Coordinates: 2891091..2891348 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJM00_RS14020	2887005..2887319	+	315	WP_009937839.1	hypothetical protein	-
BJM00_RS14025	2887615..2888826	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
BJM00_RS14035	2888953..2889612	+	660	WP_003902296.1	hypothetical protein	-
BJM00_RS14040	2889609..2890272	+	664	Protein_2648	hypothetical protein	-
BJM00_RS21695	2890269..2890546	+	278	Protein_2649	type II toxin-antitoxin system VapB family antitoxin	-
BJM00_RS14050	2890639..2891052	+	414	WP_003413164.1	PIN domain nuclease	-
BJM00_RS14055	2891091..2891348	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
BJM00_RS14060	2891345..2891722	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
BJM00_RS14065	2891738..2892112	-	375	WP_003413177.1	hypothetical protein	-
BJM00_RS14070	2892212..2892607	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
BJM00_RS14075	2892604..2892849	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
BJM00_RS14085	2893260..2893679	-	420	WP_003413190.1	A24 family peptidase	-
BJM00_RS14090	2893691..2894500	-	810	WP_003413193.1	shikimate dehydrogenase	-
BJM00_RS14095	2894497..2895750	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
BJM00_RS14100	2895743..2896255	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T67542 WP_003413174.1 NZ_CP017594:2891345-2891722 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T67542 NZ_CP017594:2891345-2891722 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT67542 WP_003413167.1 NZ_CP017594:2891091-2891348 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT67542 NZ_CP017594:2891091-2891348 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5