Detailed information of TA system TA067468

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2195756..2196459
• Replicon: chromosome
• Accession: NZ_CP017593
• Organism: Mycobacterium tuberculosis strain Beijing-like/35049

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: BJL99_RS10525
• Protein ID: WP_003409778.1
• Coordinates: 2195756..2196085 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: BJL99_RS10530
• Protein ID: WP_003409780.1
• Coordinates: 2196082..2196459 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJL99_RS10505	2192139..2193209	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
BJL99_RS10510	2193206..2193721	+	516	WP_003409718.1	flavin reductase family protein	-
BJL99_RS10515	2193718..2194779	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
BJL99_RS10520	2194776..2195546	+	771	WP_003409775.1	SDR family oxidoreductase	-
BJL99_RS10525	2195756..2196085	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BJL99_RS10530	2196082..2196459	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
BJL99_RS10535	2196456..2197046	-	591	WP_003409784.1	SEC-C domain-containing protein	-
BJL99_RS10540	2197101..2198465	+	1365	WP_003903691.1	HNH endonuclease	-
BJL99_RS10545	2198620..2199072	-	453	WP_003899095.1	lipoprotein	-
BJL99_RS10550	2199136..2199537	+	402	WP_003409869.1	hypothetical protein	-
BJL99_RS10555	2199530..2199712	-	183	WP_003409870.1	hypothetical protein	-
BJL99_RS10560	2199826..2200176	-	351	WP_003409871.1	hypothetical protein	-
BJL99_RS10565	2200187..2201089	-	903	WP_003899097.1	hypothetical protein	-
BJL99_RS10570	2201110..2201301	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T67468 WP_003409778.1 NZ_CP017593:c2196085-2195756 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T67468 NZ_CP017593:c2196085-2195756 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT67468 WP_003409780.1 NZ_CP017593:c2196459-2196082 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT67468 NZ_CP017593:c2196459-2196082 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8