Detailed information of TA system TA067459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1253064..1253632
• Replicon: chromosome
• Accession: NZ_CP017593
• Organism: Mycobacterium tuberculosis strain Beijing-like/35049

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: BJL99_RS06160
• Protein ID: WP_003405865.1
• Coordinates: 1253258..1253632 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: BJL99_RS06155
• Protein ID: WP_003405863.1
• Coordinates: 1253064..1253261 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJL99_RS06135	1249105..1249743	-	639	WP_003898730.1	lipid droplet-associated protein	-
BJL99_RS06140	1249833..1250840	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
BJL99_RS06145	1250857..1251840	-	984	WP_003898731.1	hypothetical protein	-
BJL99_RS06150	1251903..1252976	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
BJL99_RS06155	1253064..1253261	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BJL99_RS06160	1253258..1253632	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
BJL99_RS06165	1253835..1254533	+	699	WP_003898733.1	hypothetical protein	-
BJL99_RS06170	1254651..1254836	+	186	WP_003901093.1	hypothetical protein	-
BJL99_RS06175	1254763..1255038	-	276	WP_031651777.1	hypothetical protein	-
BJL99_RS06180	1255281..1255604	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
BJL99_RS06185	1255619..1256329	-	711	WP_003911399.1	hypothetical protein	-
BJL99_RS21905	1256351..1256560	+	210	WP_003911400.1	hypothetical protein	-
BJL99_RS06190	1256512..1257219	-	708	Protein_1176	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T67459 WP_003405865.1 NZ_CP017593:1253258-1253632 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T67459 NZ_CP017593:1253258-1253632 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT67459 WP_003405863.1 NZ_CP017593:1253064-1253261 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT67459 NZ_CP017593:1253064-1253261 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07