Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 855333..856042 Replicon chromosome
Accession NZ_CP017593
Organism Mycobacterium tuberculosis strain Beijing-like/35049

Toxin (Protein)


Gene name vapC Uniprot ID P9WF74
Locus tag BJL99_RS04125 Protein ID WP_003403837.1
Coordinates 855614..856042 (+) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TEX4
Locus tag BJL99_RS04120 Protein ID WP_003403834.1
Coordinates 855333..855590 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJL99_RS04100 850861..852054 + 1194 Protein_789 phosphoglycerate mutase PE_PGRS11 -
BJL99_RS21860 852465..852671 - 207 WP_164452149.1 hypothetical protein -
BJL99_RS04115 853033..855242 + 2210 Protein_791 PE family protein -
BJL99_RS04120 855333..855590 + 258 WP_003403834.1 ribbon-helix-helix protein, CopG family Antitoxin
BJL99_RS04125 855614..856042 + 429 WP_003403837.1 type II toxin-antitoxin system VapC family toxin Toxin
BJL99_RS04130 856132..856306 - 175 Protein_794 transposase -
BJL99_RS04135 856419..856664 + 246 WP_003403841.1 hypothetical protein -
BJL99_RS04140 856733..857617 - 885 WP_003403844.1 3-hydroxyisobutyrate dehydrogenase -
BJL99_RS04145 857628..858800 - 1173 WP_003403845.1 acyl-CoA dehydrogenase FadE9 -
BJL99_RS04150 858807..860339 - 1533 WP_031709363.1 CoA-acylating methylmalonate-semialdehyde dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-141)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-36)


Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15831.27 Da        Isoelectric Point: 7.5536

>T67455 WP_003403837.1 NZ_CP017593:855614-856042 [Mycobacterium tuberculosis]
MFLLDANVLLAAHRGDHPNHRTVRPWFDRLLAADDPFTVPNLVWASFLRLATNRRIFEIPSPRAEAFAFVEAVTAQPHHL
PTNPGPRHLMLLRKLCDEADASGDLIPDAVLAAIAVGHHCAVVSLDRDFARFASVRHIRPPL

Download         Length: 429 bp

>T67455 NZ_CP017593:855614-856042 [Mycobacterium tuberculosis]
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTCGCGGCTGACGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATC
GACGCATCTTCGAGATTCCGTCACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTT
CCGACGAACCCCGGTCCCAGACACCTCATGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGCGGCCATAGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCT
CGGTGCGCCACATTCGCCCGCCGCTCTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9114.28 Da        Isoelectric Point: 10.6530

>AT67455 WP_003403834.1 NZ_CP017593:855333-855590 [Mycobacterium tuberculosis]
MRTTVSISDEILAAAKRRARERGQSLGAVIEDALRREFAAAHVGGARPTVPVFDGGTGPRRGIDLTSNRALSEVLDEGLE
LNSRK

Download         Length: 258 bp

>AT67455 NZ_CP017593:855333-855590 [Mycobacterium tuberculosis]
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAGGACGCCCTTCGGCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGAGCGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDR3

Antitoxin

Source ID Structure
AlphaFold DB G0TEX4

References