Detailed information of TA system TA067436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 49116..49636
• Replicon: plasmid pDSJ10
• Accession: NZ_CP017591
• Organism: Pantoea stewartii subsp. stewartii DC283

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: H3RK38
• Locus tag: DSJ_RS25700
• Protein ID: WP_006121898.1
• Coordinates: 49116..49415 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: H3RK39
• Locus tag: DSJ_RS25705
• Protein ID: WP_006121899.1
• Coordinates: 49418..49636 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSJ_RS25680	44722..45840	+	1119	Protein_44	glucan 1,4-alpha-glucosidase	-
DSJ_RS25685	45842..46846	+	1005	WP_006121895.1	decarboxylating 6-phosphogluconate dehydrogenase	-
DSJ_RS25690	46864..48234	+	1371	WP_006121896.1	glucose-6-phosphate dehydrogenase	-
DSJ_RS25695	48252..48947	+	696	WP_006121897.1	ROK family protein	-
DSJ_RS25700	49116..49415	-	300	WP_006121898.1	CcdB family protein	Toxin
DSJ_RS25705	49418..49636	-	219	WP_006121899.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
DSJ_RS25710	50149..50409	+	261	WP_006121900.1	hypothetical protein	-
DSJ_RS25715	50612..51037	-	426	WP_006121901.1	ATP-binding protein	-
DSJ_RS25720	51039..51335	-	297	WP_044243057.1	STAS domain-containing protein	-
DSJ_RS25725	51342..52913	-	1572	WP_006117591.1	IS66 family transposase	-
DSJ_RS25730	52933..53280	-	348	WP_006118527.1	IS66 family insertion sequence element accessory protein TnpB	-
DSJ_RS25735	53277..53906	-	630	WP_044242011.1	IS66 family insertion sequence hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fliC	1..304641	304641

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-98)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10974.67 Da
• Isoelectric Point: 7.0045

>T67436 WP_006121898.1 NZ_CP017591:c49415-49116 [Pantoea stewartii subsp. stewartii DC283]
MQFDVYSHKNGKKYPYLVEVQSYLIDTPGRLMVAPLALPGEFVGSGELYPEVLVNGSKYRVVTTDIASVPVKALGDKVGD
VSQYEAAIKNAIFRLFWGF

Nucleotide

• Length: 300 bp

>T67436 NZ_CP017591:c49415-49116 [Pantoea stewartii subsp. stewartii DC283]
ATGCAATTTGACGTTTACTCACACAAGAATGGTAAAAAATATCCTTATCTTGTTGAAGTACAAAGCTACCTGATAGACAC
ACCAGGCCGTTTAATGGTCGCCCCCCTTGCTCTTCCGGGCGAGTTCGTGGGTTCAGGTGAACTTTATCCTGAGGTACTGG
TTAACGGCAGCAAGTACCGCGTTGTGACAACCGACATTGCATCTGTCCCGGTGAAAGCGTTAGGGGACAAAGTGGGTGAT
GTCAGTCAGTATGAAGCAGCAATAAAAAACGCCATTTTCAGGCTTTTTTGGGGTTTCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8516.56 Da
• Isoelectric Point: 5.1504

>AT67436 WP_006121899.1 NZ_CP017591:c49636-49418 [Pantoea stewartii subsp. stewartii DC283]
MKYRTNVTIDKEAYEIFSSAGLNLSKMVNELVTTEARRIRAERWKEENRESMKAFSDFVGEEGSLSDMYRNW

Nucleotide

• Length: 219 bp

>AT67436 NZ_CP017591:c49636-49418 [Pantoea stewartii subsp. stewartii DC283]
ATGAAATACAGAACTAACGTCACCATTGATAAAGAAGCCTACGAGATATTTTCATCTGCGGGCTTAAACCTTTCTAAAAT
GGTTAACGAGCTTGTCACTACTGAGGCTCGCAGAATCCGGGCTGAACGCTGGAAAGAAGAAAACCGTGAAAGCATGAAAG
CCTTCTCTGACTTTGTGGGAGAAGAGGGATCGCTTTCTGATATGTACAGGAACTGGTAG

Structures

Toxin

• AlphaFold DB: H3RK38

Antitoxin

• AlphaFold DB: H3RK39