Detailed information of TA system TA067435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 104435..105192
• Replicon: plasmid pDSJ09
• Accession: NZ_CP017590
• Organism: Pantoea stewartii subsp. stewartii DC283

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: DSJ_RS25290
• Protein ID: WP_006122261.1
• Coordinates: 104710..105192 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A7U5G7A5
• Locus tag: DSJ_RS25285
• Protein ID: WP_044243377.1
• Coordinates: 104435..104719 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSJ_RS25245	99764..100111	-	348	WP_006117592.1	IS66 family insertion sequence element accessory protein TnpB	-
DSJ_RS25250	100108..100737	-	630	WP_044241253.1	IS66 family insertion sequence hypothetical protein	-
DSJ_RS25255	100768..101442	-	675	WP_006122268.1	type-F conjugative transfer system secretin TraK	-
DSJ_RS25260	101429..101995	-	567	WP_006122267.1	type IV conjugative transfer system protein TraE	-
DSJ_RS25265	102017..102322	-	306	WP_006122266.1	type IV conjugative transfer system protein TraL	-
DSJ_RS25270	102341..102745	-	405	WP_006122265.1	conjugal transfer protein TraA	-
DSJ_RS25275	103219..103569	+	351	WP_044243379.1	hypothetical protein	-
DSJ_RS25280	103556..103804	-	249	WP_006122263.1	TraY domain-containing protein	-
DSJ_RS28145	103778..103924	-	147	Protein_108	TraY domain-containing protein	-
DSJ_RS25285	104435..104719	+	285	WP_044243377.1	DUF1778 domain-containing protein	Antitoxin
DSJ_RS25290	104710..105192	+	483	WP_006122261.1	GNAT family N-acetyltransferase	Toxin
DSJ_RS25295	105272..105937	-	666	WP_044243375.1	LuxR C-terminal-related transcriptional regulator	-
DSJ_RS25300	105921..106334	+	414	Protein_112	DUF4113 domain-containing protein	-
DSJ_RS25305	106542..106769	-	228	WP_020314634.1	hypothetical protein	-
DSJ_RS25310	106814..107440	-	627	WP_044243371.1	ParA family protein	-
DSJ_RS25315	107920..108540	+	621	WP_006122260.1	recombinase family protein	-
DSJ_RS25320	108944..109417	-	474	Protein_116	IS91 family transposase	-
DSJ_RS25325	109503..110126	+	624	WP_071988728.1	IS66 family insertion sequence hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..132938	132938

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 20654.05 Da
• Isoelectric Point: 13.0041

>T67435 WP_006122261.1 NZ_CP017590:104710-105192 [Pantoea stewartii subsp. stewartii DC283]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
K

Nucleotide

• Length: 483 bp

>T67435 NZ_CP017590:104710-105192 [Pantoea stewartii subsp. stewartii DC283]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 12194.55 Da
• Isoelectric Point: 12.7731

>AT67435 WP_044243377.1 NZ_CP017590:104435-104719 [Pantoea stewartii subsp. stewartii DC283]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKK

Nucleotide

• Length: 285 bp

>AT67435 NZ_CP017590:104435-104719 [Pantoea stewartii subsp. stewartii DC283]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Antitoxin

• AlphaFold DB: A0A7U5G7A5