Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 527062..528572 Replicon chromosome
Accession NZ_CP017578
Organism Sphingomonas melonis TY

Toxin (Protein)


Gene name HipA1 Uniprot ID A0A175Y3U4
Locus tag BJP26_RS02620 Protein ID WP_017980415.1
Coordinates 527062..528327 (-) Length 422 a.a.

Antitoxin (Protein)


Gene name HipB1 Uniprot ID A0A175Y469
Locus tag BJP26_RS02625 Protein ID WP_017980416.1
Coordinates 528324..528572 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJP26_RS02600 522645..523544 + 900 WP_017980411.1 HNH endonuclease -
BJP26_RS19430 523890..524228 + 339 Protein_530 glutathione S-transferase C-terminal domain-containing protein -
BJP26_RS02610 524346..525230 - 885 WP_017980413.1 LysR family transcriptional regulator -
BJP26_RS02615 525387..526844 + 1458 WP_017980414.1 MFS transporter -
BJP26_RS02620 527062..528327 - 1266 WP_017980415.1 type II toxin-antitoxin system HipA family toxin Toxin
BJP26_RS02625 528324..528572 - 249 WP_017980416.1 helix-turn-helix transcriptional regulator Antitoxin
BJP26_RS02630 529018..530289 + 1272 WP_017980418.1 dicarboxylate/amino acid:cation symporter -
BJP26_RS02635 530471..532252 - 1782 WP_017980419.1 hypothetical protein -
BJP26_RS02640 532312..532743 + 432 WP_017980420.1 GtrA family protein -
BJP26_RS02645 532747..533475 + 729 WP_017980421.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-75)


Sequences

Toxin        


Download         Length: 422 a.a.        Molecular weight: 46236.14 Da        Isoelectric Point: 6.4978

>T67428 WP_017980415.1 NZ_CP017578:c528327-527062 [Sphingomonas melonis TY]
MILPVYFEEHLVGEVHEVVDGPRFVYAASWTEDRQAFPISITMPLGEREVEPARFLTWASNLLPEAEQLVVIARQLGVAQ
TDILALLEAIGRDTAGALSFGAPGLTRTERWQRIETEDDLERIIEELPRKPFLAGDDGVSMSLAGVQTKLAVACDADGRI
CIPLDGSPSTHILKPDSERLHGSVQNEAWCLTLARLCGLTVPPVTTGVAGGRRYFLIPRYDRVPDGAQWRRLHQEDFCQA
LGKPPFAKYERNRTGVRGPTVADMMNLVRRQALGPDALRLFDAIVFNVLACNTDAHAKNYSILIPAGSRVRTAPLYDVMC
AEVFDGVTHNLAQTVADKNRGNYLKRRHWLRLAAACGLGAAPTIRRVRALATRVLANVDRARMDVEAMPAGGDATLPMIV
DAVSERCRAILAGLDDMEGVK

Download         Length: 1266 bp

>T67428 NZ_CP017578:c528327-527062 [Sphingomonas melonis TY]
ATGATCCTCCCGGTCTATTTTGAGGAGCATCTGGTCGGCGAAGTCCATGAGGTCGTTGACGGACCGCGGTTCGTGTACGC
AGCATCGTGGACGGAGGACCGACAGGCCTTCCCCATCTCGATCACCATGCCGCTGGGCGAGCGCGAGGTGGAGCCTGCTC
GGTTCCTGACCTGGGCCAGCAACCTGCTTCCCGAGGCGGAGCAGCTCGTGGTCATCGCCCGTCAACTCGGTGTCGCGCAG
ACAGACATCCTCGCCCTGCTGGAGGCGATCGGCCGCGACACCGCCGGCGCGCTTTCCTTTGGTGCGCCGGGGCTGACCCG
CACAGAGCGCTGGCAGCGGATCGAGACAGAGGACGATCTCGAACGCATCATCGAGGAGCTTCCCCGCAAGCCGTTCCTCG
CGGGCGATGACGGCGTGTCGATGTCGCTGGCGGGCGTCCAGACCAAGCTGGCGGTGGCCTGCGACGCCGACGGCCGGATC
TGCATTCCGCTCGACGGGTCGCCCTCGACCCATATCCTCAAGCCCGACAGCGAACGCCTCCACGGCAGCGTCCAGAACGA
GGCTTGGTGCCTGACGCTGGCTAGGCTCTGCGGATTGACGGTGCCGCCGGTTACGACCGGAGTGGCGGGCGGAAGGCGCT
ACTTCCTGATCCCGCGCTACGACCGCGTGCCAGACGGAGCGCAATGGCGTCGGCTGCATCAGGAGGACTTTTGCCAAGCG
CTGGGTAAGCCACCGTTCGCCAAGTACGAGCGGAACAGAACGGGCGTCCGTGGCCCGACCGTCGCCGACATGATGAACCT
CGTCCGGCGCCAGGCGCTCGGTCCTGATGCACTGCGGCTGTTCGACGCCATCGTCTTCAACGTACTGGCGTGCAACACCG
ATGCCCATGCCAAGAACTACTCGATCCTGATCCCCGCAGGATCGCGGGTGCGCACCGCTCCCCTATACGACGTGATGTGC
GCGGAGGTGTTCGATGGCGTGACCCACAATCTCGCGCAGACGGTCGCCGACAAGAACCGCGGCAACTATCTGAAGCGAAG
GCACTGGCTGCGCCTCGCCGCCGCCTGCGGACTCGGCGCTGCACCGACCATCCGCCGCGTCCGCGCGCTGGCGACCCGGG
TGCTCGCCAATGTCGATCGGGCGCGGATGGATGTCGAAGCCATGCCCGCAGGCGGTGACGCCACCCTGCCAATGATCGTC
GACGCCGTGTCGGAGCGGTGCCGCGCTATCCTCGCTGGTCTCGATGACATGGAAGGCGTGAAGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8897.16 Da        Isoelectric Point: 5.7710

>AT67428 WP_017980416.1 NZ_CP017578:c528572-528324 [Sphingomonas melonis TY]
MDQADTENDAAWFSRRFGALVRSRRQQMGLSLEDLATVAGVGIRFIHELEKGKPTCQIGRALVVAGLVGLDPVTLLEQQS
AS

Download         Length: 249 bp

>AT67428 NZ_CP017578:c528572-528324 [Sphingomonas melonis TY]
ATGGATCAGGCTGACACGGAAAACGATGCCGCATGGTTTTCGCGCCGCTTCGGTGCCCTCGTCCGCTCGCGCCGACAGCA
GATGGGGCTAAGTCTCGAGGATCTGGCGACCGTCGCGGGCGTGGGTATCCGGTTCATCCACGAATTGGAGAAGGGCAAGC
CGACCTGCCAGATCGGCCGCGCCCTCGTCGTCGCCGGGCTGGTCGGGCTGGACCCGGTCACCCTGCTTGAACAGCAAAGC
GCCTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A175Y3U4

Antitoxin

Source ID Structure
AlphaFold DB A0A175Y469

References