Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4433770..4434412 Replicon chromosome
Accession NZ_CP017577
Organism Bacillus thuringiensis strain SCG04-02

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BJG91_RS25745 Protein ID WP_000635963.1
Coordinates 4434062..4434412 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BJG91_RS25740 Protein ID WP_000004570.1
Coordinates 4433770..4434057 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJG91_RS25715 4429087..4430049 + 963 WP_000961165.1 UV DNA damage repair endonuclease UvsE -
BJG91_RS25720 4430042..4430614 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
BJG91_RS25725 4430708..4431067 + 360 WP_000583416.1 holo-ACP synthase -
BJG91_RS25730 4431224..4432174 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
BJG91_RS25735 4432292..4433461 + 1170 WP_000390615.1 alanine racemase -
BJG91_RS25740 4433770..4434057 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BJG91_RS25745 4434062..4434412 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BJG91_RS25750 4434480..4436648 + 2169 WP_000426242.1 RNA-binding transcriptional accessory protein -
BJG91_RS25755 4436706..4436822 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BJG91_RS25760 4437018..4437476 + 459 WP_000344234.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T67427 WP_000635963.1 NZ_CP017577:4434062-4434412 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T67427 NZ_CP017577:4434062-4434412 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT67427 WP_000004570.1 NZ_CP017577:4433770-4434057 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT67427 NZ_CP017577:4433770-4434057 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References