Detailed information of TA system TA067424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 187713..188635
• Replicon: plasmid pl2WSM5005
• Accession: NZ_CP017565
• Organism: Paraburkholderia sprentiae WSM5005

Toxin (Protein)

• Gene name: PrcT
• Uniprot ID: -
• Locus tag: BJG93_RS33795
• Protein ID: WP_154677418.1
• Coordinates: 188165..188635 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A1I9YW75
• Locus tag: BJG93_RS33790
• Protein ID: WP_027193709.1
• Coordinates: 187713..188168 (+)
• Length: 152 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJG93_RS33770	185478..185720	+	243	WP_027196623.1	type II toxin-antitoxin system ParD family antitoxin	-
BJG93_RS33775	185717..186019	+	303	WP_027196622.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BJG93_RS37250	186078..186414	+	337	Protein_184	hypothetical protein	-
BJG93_RS33780	186829..187161	+	333	WP_071336754.1	hypothetical protein	-
BJG93_RS37670	187412..187570	+	159	WP_167544181.1	hypothetical protein	-
BJG93_RS33790	187713..188168	+	456	WP_027193709.1	DUF2384 domain-containing protein	Antitoxin
BJG93_RS33795	188165..188635	+	471	WP_154677418.1	RES family NAD+ phosphorylase	Toxin
BJG93_RS33800	188643..188837	+	195	WP_027196619.1	hypothetical protein	-
BJG93_RS33805	189816..190094	+	279	WP_027196618.1	HU family DNA-binding protein	-
BJG93_RS33810	190091..191248	+	1158	WP_027196617.1	DUF1016 family protein	-
BJG93_RS33815	191668..192681	-	1014	WP_027196616.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..438974	438974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (4-134)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (35-93)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17092.50 Da
• Isoelectric Point: 4.9119

>T67424 WP_154677418.1 NZ_CP017565:188165-188635 [Paraburkholderia sprentiae WSM5005]
MMVLWRISNYADLKGIGGQRAAGRWHFAGQPVVYLAEHPAAALLEILVHLEIGSPADLPDTYQLLRVEVADDVTIAEMTD
DGIPANWRDDMAWSQDAGTEWLGAGGSVLLKVPSAVVPYAHNYLMNPEHEDAGHLTISMAIQVRHDSRILKLLSKG

Nucleotide

• Length: 471 bp

>T67424 NZ_CP017565:188165-188635 [Paraburkholderia sprentiae WSM5005]
CTGATGGTCCTGTGGAGAATCAGTAACTACGCCGACCTGAAAGGCATTGGTGGCCAGCGGGCAGCGGGCCGATGGCATTT
CGCCGGCCAGCCGGTAGTGTATCTGGCCGAACATCCCGCGGCCGCCCTTCTCGAGATTCTGGTACATCTGGAAATCGGCA
GCCCCGCTGATCTACCCGATACGTACCAGCTGCTCAGGGTTGAAGTGGCAGACGACGTAACGATTGCGGAGATGACCGAC
GACGGCATCCCCGCCAACTGGCGGGACGATATGGCGTGGTCCCAGGATGCCGGGACTGAATGGCTGGGTGCCGGCGGCAG
CGTGCTGCTTAAGGTGCCAAGCGCAGTCGTTCCTTACGCGCACAACTACCTGATGAATCCCGAACACGAAGACGCGGGTC
ACCTCACGATCAGCATGGCAATCCAGGTCCGACACGATTCCCGTATTCTGAAATTGCTGTCAAAAGGGTAG

Antitoxin

Protein

• Length: 152 a.a.
• Molecular weight: 16681.99 Da
• Isoelectric Point: 9.8528

>AT67424 WP_027193709.1 NZ_CP017565:187713-188168 [Paraburkholderia sprentiae WSM5005]
MITVSFKPSGAGNPRREEFALLQRLLDAPVRSGSDLAELATERVGVAVIDRLAEHGLKADELSFIIPRRTLTHRRQQHER
LSTEESDKAIRLAKIVAQATSAFGNAGKAMLWLRNSQKRFGGRTALDMASTEHGARLVEEALVQLDEGYFA

Nucleotide

• Length: 456 bp

>AT67424 NZ_CP017565:187713-188168 [Paraburkholderia sprentiae WSM5005]
ATGATCACTGTCTCTTTCAAGCCAAGCGGAGCGGGCAACCCGCGCCGGGAAGAGTTCGCCCTGTTGCAGCGGCTGCTTGA
TGCGCCCGTGCGCAGCGGTTCCGACCTTGCAGAGCTCGCGACCGAACGGGTCGGCGTCGCCGTGATCGACCGTCTTGCAG
AGCACGGACTGAAGGCCGACGAACTGTCTTTCATCATCCCCCGGCGGACGCTGACACACCGTCGGCAACAGCACGAGCGA
CTCTCGACGGAGGAGTCAGACAAGGCGATTCGTCTCGCCAAGATCGTCGCCCAGGCGACCTCAGCCTTTGGCAATGCCGG
GAAGGCAATGCTGTGGCTGCGAAATTCCCAGAAGCGGTTCGGTGGGAGGACCGCGCTCGATATGGCGAGCACCGAGCACG
GCGCCCGGTTGGTCGAGGAGGCGCTCGTCCAGCTTGACGAAGGGTACTTTGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1I9YW75