Detailed information of TA system TA067414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1615052..1615692
• Replicon: chromosome
• Accession: NZ_CP017552
• Organism: Mannheimia haemolytica strain 1282

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A378NFX7
• Locus tag: BG607_RS08265
• Protein ID: WP_006250075.1
• Coordinates: 1615052..1615234 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378NFW9
• Locus tag: BG607_RS08270
• Protein ID: WP_006250074.1
• Coordinates: 1615273..1615692 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG607_RS08220	1610189..1610488	+	300	WP_006247810.1	XRE family transcriptional regulator	-
BG607_RS08230	1610605..1611645	+	1041	WP_015587043.1	IS481 family transposase	-
BG607_RS14005	1611705..1611863	-	159	WP_006247809.1	hypothetical protein	-
BG607_RS14010	1611873..1612940	-	1068	WP_006253284.1	hypothetical protein	-
BG607_RS08245	1612961..1613161	-	201	WP_006248825.1	helix-turn-helix domain-containing protein	-
BG607_RS08250	1613292..1613975	+	684	WP_006248824.1	helix-turn-helix transcriptional regulator	-
BG607_RS08255	1614050..1614430	+	381	WP_006248823.1	hypothetical protein	-
BG607_RS08260	1614435..1614920	+	486	WP_015484250.1	hypothetical protein	-
BG607_RS08265	1615052..1615234	+	183	WP_006250075.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG607_RS08270	1615273..1615692	+	420	WP_006250074.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG607_RS08275	1616028..1616672	+	645	WP_006253274.1	tyrosine-type recombinase/integrase	-
BG607_RS08285	1616810..1617816	+	1007	Protein_1608	IS481 family transposase	-
BG607_RS08290	1617883..1619289	-	1407	WP_006253513.1	YdgA family protein	-
BG607_RS08295	1619339..1620145	-	807	WP_006248821.1	tryptophan synthase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1606248..1673657	67409
	-	flank	IS/Tn	-	-	1610605..1611645	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-96)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.03 Da
• Isoelectric Point: 10.3446

>T67414 WP_006250075.1 NZ_CP017552:1615052-1615234 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T67414 NZ_CP017552:1615052-1615234 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15938.30 Da
• Isoelectric Point: 4.6963

>AT67414 WP_006250074.1 NZ_CP017552:1615273-1615692 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Nucleotide

• Length: 420 bp

>AT67414 NZ_CP017552:1615273-1615692 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A378NFX7

Antitoxin

• AlphaFold DB: A0A378NFW9