Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 1923134..1923673 Replicon chromosome
Accession NZ_CP017551
Organism Mannheimia haemolytica strain 1287

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZYA9
Locus tag BG585_RS10025 Protein ID WP_006247804.1
Coordinates 1923404..1923673 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A547E920
Locus tag BG585_RS10020 Protein ID WP_006247803.1
Coordinates 1923134..1923400 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG585_RS09965 1918534..1919169 - 636 Protein_1939 transposase -
BG585_RS09975 1919270..1919980 - 711 Protein_1940 tyrosine-type recombinase/integrase -
BG585_RS09980 1919884..1920207 - 324 WP_006253316.1 helix-turn-helix domain-containing protein -
BG585_RS09985 1920288..1920455 - 168 WP_006253314.1 DNA cytosine methyltransferase -
BG585_RS09990 1920463..1920837 - 375 WP_006247798.1 hypothetical protein -
BG585_RS09995 1920987..1921214 + 228 Protein_1944 phage terminase large subunit family protein -
BG585_RS10000 1921239..1921634 + 396 WP_006247799.1 phage tail protein -
BG585_RS10005 1921666..1922307 + 642 WP_006247800.1 hypothetical protein -
BG585_RS10010 1922390..1922791 + 402 WP_006247801.1 hypothetical protein -
BG585_RS10015 1922836..1923066 + 231 WP_006247802.1 DUF4035 domain-containing protein -
BG585_RS10020 1923134..1923400 + 267 WP_006247803.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BG585_RS10025 1923404..1923673 + 270 WP_006247804.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
BG585_RS10030 1923748..1924011 + 264 WP_006247805.1 hypothetical protein -
BG585_RS10035 1924071..1924781 + 711 WP_006253312.1 tape measure protein -
BG585_RS10040 1924759..1925049 + 291 Protein_1953 C40 family peptidase -
BG585_RS10045 1925039..1925758 + 720 WP_006247806.1 preprotein translocase subunit YajC -
BG585_RS10050 1925980..1926606 + 627 WP_006247807.1 tail assembly protein -
BG585_RS10055 1926655..1927266 + 612 WP_006253310.1 hypothetical protein -
BG585_RS10060 1927377..1928063 + 687 WP_006247808.1 hypothetical protein -
BG585_RS14395 1928073..1928231 + 159 WP_006247809.1 hypothetical protein -
BG585_RS10065 1928240..1928539 - 300 WP_006247810.1 XRE family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1910521..1934727 24206
- flank IS/Tn - - 1918477..1919169 692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-75)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10278.90 Da        Isoelectric Point: 6.2121

>T67407 WP_006247804.1 NZ_CP017551:1923404-1923673 [Mannheimia haemolytica]
MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKYRDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVR
LGSHAELFG

Download         Length: 270 bp

>T67407 NZ_CP017551:1923404-1923673 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9913.38 Da        Isoelectric Point: 7.0082

>AT67407 WP_006247803.1 NZ_CP017551:1923134-1923400 [Mannheimia haemolytica]
MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAKTKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEA
FHKAMLAE

Download         Length: 267 bp

>AT67407 NZ_CP017551:1923134-1923400 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZYA9

Antitoxin

Source ID Structure
AlphaFold DB A0A547E920

References