Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1132706..1133346 Replicon chromosome
Accession NZ_CP017551
Organism Mannheimia haemolytica strain 1287

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG585_RS05800 Protein ID WP_006250075.1
Coordinates 1133164..1133346 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NFW9
Locus tag BG585_RS05795 Protein ID WP_006250074.1
Coordinates 1132706..1133125 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG585_RS05770 1128253..1129059 + 807 WP_006248821.1 tryptophan synthase subunit alpha -
BG585_RS05775 1129109..1130515 + 1407 WP_006253513.1 YdgA family protein -
BG585_RS05780 1130582..1131588 - 1007 Protein_1125 IS481 family transposase -
BG585_RS05790 1131726..1132370 - 645 WP_006253274.1 tyrosine-type recombinase/integrase -
BG585_RS05795 1132706..1133125 - 420 WP_006250074.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG585_RS05800 1133164..1133346 - 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG585_RS05805 1133478..1133963 - 486 WP_015484250.1 hypothetical protein -
BG585_RS05810 1133968..1134348 - 381 WP_006248823.1 hypothetical protein -
BG585_RS05815 1134423..1135102 - 680 Protein_1131 helix-turn-helix transcriptional regulator -
BG585_RS05820 1135233..1135433 + 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG585_RS05825 1135454..1135654 + 201 Protein_1133 hypothetical protein -
BG585_RS14280 1135818..1135988 + 171 WP_006250083.1 hypothetical protein -
BG585_RS05830 1135963..1136832 + 870 WP_006250084.1 replication protein -
BG585_RS05835 1136829..1138190 + 1362 WP_006250085.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1075157..1186961 111804


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T67405 WP_006250075.1 NZ_CP017551:c1133346-1133164 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T67405 NZ_CP017551:c1133346-1133164 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15938.30 Da        Isoelectric Point: 4.6963

>AT67405 WP_006250074.1 NZ_CP017551:c1133125-1132706 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Download         Length: 420 bp

>AT67405 NZ_CP017551:c1133125-1132706 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NFW9

References