Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 2183839..2184361 Replicon chromosome
Accession NZ_CP017550
Organism Mannheimia haemolytica strain 1309

Toxin (Protein)


Gene name - Uniprot ID A0A249A107
Locus tag BG589_RS11380 Protein ID WP_006249269.1
Coordinates 2184065..2184361 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A249A190
Locus tag BG589_RS11375 Protein ID WP_006249270.1
Coordinates 2183839..2184081 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG589_RS11350 2179677..2180766 - 1090 Protein_2198 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
BG589_RS11355 2180940..2181581 - 642 WP_006249274.1 orotate phosphoribosyltransferase -
BG589_RS11360 2181704..2182546 - 843 WP_006249273.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
BG589_RS11365 2182606..2183181 + 576 WP_006249272.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
BG589_RS11370 2183159..2183827 - 669 WP_006249271.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
BG589_RS11375 2183839..2184081 - 243 WP_006249270.1 CopG family transcriptional regulator Antitoxin
BG589_RS11380 2184065..2184361 - 297 WP_006249269.1 BrnT family toxin Toxin
BG589_RS11385 2184488..2187400 - 2913 WP_006249267.1 RNA polymerase-associated protein RapA -
BG589_RS11390 2187561..2187860 - 300 WP_006249265.1 hypothetical protein -
BG589_RS11395 2187981..2189306 - 1326 WP_006249264.1 deoxyguanosinetriphosphate triphosphohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(15-90)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 12009.89 Da        Isoelectric Point: 7.9454

>T67400 WP_006249269.1 NZ_CP017550:c2184361-2184065 [Mannheimia haemolytica]
MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRL
ISVRRSRKKEISYYEKYH

Download         Length: 297 bp

>T67400 NZ_CP017550:c2184361-2184065 [Mannheimia haemolytica]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTGTTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAATA
TGGTATTGATTTTGAACAGGCAAAGTTGCTTTGGGAAGATGAACGAAAAGTTGTACTGGAGGCTTGCATTGAACCCGAAT
TACGCTATTTCCTGATTGGCAAGATTTATGATAAATATTGGACAGCGTTTTTTACACCAAGAAATGATGTTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATCTCATACTATGAGAAATACCATTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9346.79 Da        Isoelectric Point: 7.1665

>AT67400 WP_006249270.1 NZ_CP017550:c2184081-2183839 [Mannheimia haemolytica]
MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFPNWMVEALDKQARHLGVPRQALIKMWIAEKLPVQSPR

Download         Length: 243 bp

>AT67400 NZ_CP017550:c2184081-2183839 [Mannheimia haemolytica]
ATGAGAAATACCATTAGTGCCGAAGAATTAGAACGTATCTTCGATGAAGGCAAAGAAGATATTTTACAATACTGCGATCT
TTCTACCGCTCATCGCCCGGGGCAAATGAAAAGGGTGAATGTTGATTTCCCAAATTGGATGGTAGAAGCGTTAGATAAGC
AAGCCAGACATTTAGGTGTGCCTCGCCAAGCCTTGATTAAAATGTGGATTGCGGAAAAATTACCGGTTCAATCACCTAGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A107

Antitoxin

Source ID Structure
AlphaFold DB A0A249A190

References