Detailed information of TA system TA067399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1870449..1871089
• Replicon: chromosome
• Accession: NZ_CP017550
• Organism: Mannheimia haemolytica strain 1309

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A378NFX7
• Locus tag: BG589_RS09695
• Protein ID: WP_006250075.1
• Coordinates: 1870907..1871089 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378NFW9
• Locus tag: BG589_RS09690
• Protein ID: WP_006250074.1
• Coordinates: 1870449..1870868 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG589_RS09665	1865996..1866802	+	807	WP_006248821.1	tryptophan synthase subunit alpha	-
BG589_RS09670	1866852..1868258	+	1407	WP_006253513.1	YdgA family protein	-
BG589_RS09675	1868325..1869331	-	1007	Protein_1862	IS481 family transposase	-
BG589_RS09685	1869469..1870113	-	645	WP_006253274.1	tyrosine-type recombinase/integrase	-
BG589_RS09690	1870449..1870868	-	420	WP_006250074.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG589_RS09695	1870907..1871089	-	183	WP_006250075.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG589_RS09700	1871221..1871706	-	486	WP_015484250.1	hypothetical protein	-
BG589_RS09705	1871711..1872091	-	381	WP_006248823.1	hypothetical protein	-
BG589_RS09710	1872166..1872849	-	684	WP_006248824.1	helix-turn-helix transcriptional regulator	-
BG589_RS09715	1872980..1873180	+	201	WP_006248825.1	helix-turn-helix domain-containing protein	-
BG589_RS14400	1873201..1874268	+	1068	WP_006253284.1	hypothetical protein	-
BG589_RS14405	1874278..1874436	+	159	WP_006247809.1	hypothetical protein	-
BG589_RS09730	1874496..1875536	-	1041	WP_015587043.1	IS481 family transposase	-
BG589_RS09740	1875653..1875952	-	300	WP_006247810.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1810717..1931468	120751
	-	flank	IS/Tn	-	-	1874496..1875536	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-96)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.03 Da
• Isoelectric Point: 10.3446

>T67399 WP_006250075.1 NZ_CP017550:c1871089-1870907 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T67399 NZ_CP017550:c1871089-1870907 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15938.30 Da
• Isoelectric Point: 4.6963

>AT67399 WP_006250074.1 NZ_CP017550:c1870868-1870449 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Nucleotide

• Length: 420 bp

>AT67399 NZ_CP017550:c1870868-1870449 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A378NFX7

Antitoxin

• AlphaFold DB: A0A378NFW9