Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1511225..1511873 Replicon chromosome
Accession NZ_CP017550
Organism Mannheimia haemolytica strain 1309

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag BG589_RS07875 Protein ID WP_006251221.1
Coordinates 1511697..1511873 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag BG589_RS07870 Protein ID WP_006251222.1
Coordinates 1511225..1511641 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG589_RS07830 1507509..1507943 + 435 WP_006251230.1 hypothetical protein -
BG589_RS07835 1507958..1508947 + 990 WP_006251229.1 encapsulin -
BG589_RS14365 1508959..1509228 + 270 WP_020849873.1 hypothetical protein -
BG589_RS07845 1509231..1509608 + 378 WP_006251227.1 hypothetical protein -
BG589_RS07850 1509608..1509952 + 345 WP_006251226.1 hypothetical protein -
BG589_RS07855 1509954..1510322 + 369 WP_006251225.1 hypothetical protein -
BG589_RS07860 1510319..1510699 + 381 WP_006251224.1 hypothetical protein -
BG589_RS07865 1510703..1511185 + 483 WP_006251223.1 phage tail protein -
BG589_RS07870 1511225..1511641 - 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG589_RS07875 1511697..1511873 - 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
BG589_RS07880 1511969..1512640 + 672 WP_006251220.1 hypothetical protein -
BG589_RS07885 1512655..1512888 - 234 WP_020824316.1 hypothetical protein -
BG589_RS07890 1513057..1513515 + 459 WP_006251218.1 hypothetical protein -
BG589_RS07900 1514060..1516555 + 2496 WP_006251216.1 phage tail length tape measure family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1453648..1528548 74900


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T67398 WP_006251221.1 NZ_CP017550:c1511873-1511697 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T67398 NZ_CP017550:c1511873-1511697 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT67398 WP_006251222.1 NZ_CP017550:c1511641-1511225 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT67398 NZ_CP017550:c1511641-1511225 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References