Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 509260..509849 Replicon chromosome
Accession NZ_CP017550
Organism Mannheimia haemolytica strain 1309

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag BG589_RS02565 Protein ID WP_006248516.1
Coordinates 509260..509592 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag BG589_RS02570 Protein ID WP_006248517.1
Coordinates 509589..509849 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG589_RS02525 504455..505075 - 621 WP_006248508.1 dTMP kinase -
BG589_RS02530 505086..506120 - 1035 WP_006248509.1 endolytic transglycosylase MltG -
BG589_RS02550 506700..507227 + 528 WP_006248512.1 inorganic diphosphatase -
BG589_RS02555 507283..508038 + 756 WP_006248513.1 M48 family metallopeptidase -
BG589_RS02560 508102..509133 - 1032 WP_006248514.1 tryptophan--tRNA ligase -
BG589_RS02565 509260..509592 - 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BG589_RS02570 509589..509849 - 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BG589_RS02575 509919..510587 - 669 WP_006248518.1 phosphoglycolate phosphatase -
BG589_RS02580 510674..511471 - 798 WP_006248519.1 prolipoprotein diacylglyceryl transferase -
BG589_RS02585 511479..512267 - 789 WP_006248520.1 sulfite exporter TauE/SafE family protein -
BG589_RS02590 512269..512913 - 645 WP_006248521.1 RNA pyrophosphohydrolase -
BG589_RS02595 513525..514670 + 1146 WP_006248526.1 FMN-dependent L-lactate dehydrogenase LldD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T67395 WP_006248516.1 NZ_CP017550:c509592-509260 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

>T67395 NZ_CP017550:c509592-509260 [Mannheimia haemolytica]
ATGAAACGTGGCGATATTTACTGGCTCGATCTTGATCCAACCTTAGGACACGAACAATCAGGCTTCCGCCCCGTTGTAAT
TGTTTCCGCAACTGCATTTAATCTAGCCAGTAAACTGCCTGTGGTTGTGCCGATTACTAACGGTGGAGCTTTTGCTGAAA
GACTGGGCTTTGCCGTTTCATTAAAAGATGCCGGCTTAAAAACAACAGGGATTATTCGATGCGATCAACCTCGCACAGTC
GATATTGCACAACGCCATGGAAAATTTATCGAAGCCTTACCCGAAAGCCTACTTGATGAAATTATGGCAAGGGTTTCAGT
GATTTTTGAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT67395 WP_006248517.1 NZ_CP017550:c509849-509589 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp

>AT67395 NZ_CP017550:c509849-509589 [Mannheimia haemolytica]
ATGCCAACAATCGCATTACGTCAGGTCGGTGGCTCGGTTGCAATGGTGATTCCGCCTGCTTTTTTAGAGGCACTCAAGCT
CTCCGTCGGTAGCAAAGTGGATTTGTCTTTAAACGACAACCAACTCGTGATTAATCCTGCCCGCCCAAAAGTGAAATACA
GCTTAGAGGAATTATTAAGACAATCCGATTTTGAGGCAGCAAGGAATGAAGAAAGCCAATCGTGGTTATCCGAGCCGGCA
ATAGGAAAAGAAATTTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZY19

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References