Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1611030..1611670 Replicon chromosome
Accession NZ_CP017549
Organism Mannheimia haemolytica strain 1318

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG602_RS08240 Protein ID WP_006250075.1
Coordinates 1611030..1611212 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NFW9
Locus tag BG602_RS08245 Protein ID WP_006250074.1
Coordinates 1611251..1611670 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG602_RS08195 1606577..1606870 - 294 WP_006250050.1 hypothetical protein -
BG602_RS08200 1607014..1607403 + 390 WP_015484247.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG602_RS08205 1607375..1607674 + 300 WP_006247810.1 XRE family transcriptional regulator -
BG602_RS14020 1607683..1607841 - 159 WP_006247809.1 hypothetical protein -
BG602_RS14025 1607851..1608918 - 1068 WP_006253284.1 hypothetical protein -
BG602_RS08220 1608939..1609139 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG602_RS08225 1609270..1609953 + 684 WP_006248824.1 helix-turn-helix transcriptional regulator -
BG602_RS08230 1610028..1610408 + 381 WP_006248823.1 hypothetical protein -
BG602_RS08235 1610413..1610898 + 486 WP_015484250.1 hypothetical protein -
BG602_RS08240 1611030..1611212 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG602_RS08245 1611251..1611670 + 420 WP_006250074.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG602_RS08250 1612006..1612650 + 645 WP_006253274.1 tyrosine-type recombinase/integrase -
BG602_RS08260 1612788..1613828 + 1041 WP_096864247.1 IS481 family transposase -
BG602_RS08265 1613895..1615301 - 1407 WP_006253513.1 YdgA family protein -
BG602_RS08270 1615351..1616157 - 807 WP_006248821.1 tryptophan synthase subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1612788..1613828 1040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T67390 WP_006250075.1 NZ_CP017549:1611030-1611212 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T67390 NZ_CP017549:1611030-1611212 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15938.30 Da        Isoelectric Point: 4.6963

>AT67390 WP_006250074.1 NZ_CP017549:1611251-1611670 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Download         Length: 420 bp

>AT67390 NZ_CP017549:1611251-1611670 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NFW9

References