Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 1961306..1961845 Replicon chromosome
Accession NZ_CP017548
Organism Mannheimia haemolytica strain 1319

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZYA9
Locus tag BG580_RS10330 Protein ID WP_006247804.1
Coordinates 1961576..1961845 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A547E920
Locus tag BG580_RS10325 Protein ID WP_006247803.1
Coordinates 1961306..1961572 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG580_RS10270 1956706..1957341 - 636 Protein_1997 transposase -
BG580_RS10280 1957442..1958152 - 711 Protein_1998 tyrosine-type recombinase/integrase -
BG580_RS10285 1958056..1958379 - 324 WP_006253316.1 helix-turn-helix domain-containing protein -
BG580_RS10290 1958460..1958627 - 168 WP_006253314.1 DNA cytosine methyltransferase -
BG580_RS10295 1958635..1959009 - 375 WP_006247798.1 hypothetical protein -
BG580_RS10300 1959159..1959386 + 228 Protein_2002 phage terminase large subunit family protein -
BG580_RS10305 1959411..1959806 + 396 WP_006247799.1 phage tail protein -
BG580_RS10310 1959838..1960479 + 642 WP_006247800.1 hypothetical protein -
BG580_RS10315 1960562..1960963 + 402 WP_006247801.1 hypothetical protein -
BG580_RS10320 1961008..1961238 + 231 WP_006247802.1 DUF4035 domain-containing protein -
BG580_RS10325 1961306..1961572 + 267 WP_006247803.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BG580_RS10330 1961576..1961845 + 270 WP_006247804.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
BG580_RS10335 1961920..1962183 + 264 WP_006247805.1 hypothetical protein -
BG580_RS10340 1962243..1962953 + 711 WP_006253312.1 tape measure protein -
BG580_RS10345 1962931..1963221 + 291 Protein_2011 C40 family peptidase -
BG580_RS10350 1963211..1963930 + 720 WP_006247806.1 preprotein translocase subunit YajC -
BG580_RS10355 1964152..1964778 + 627 WP_006247807.1 tail assembly protein -
BG580_RS10360 1964827..1965438 + 612 WP_006253310.1 hypothetical protein -
BG580_RS10365 1965549..1966235 + 687 WP_006247808.1 hypothetical protein -
BG580_RS14700 1966245..1966403 + 159 WP_006247809.1 hypothetical protein -
BG580_RS10370 1966412..1966711 - 300 WP_006247810.1 XRE family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1947399..1970247 22848
- flank IS/Tn - - 1956649..1957341 692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-75)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10278.90 Da        Isoelectric Point: 6.2121

>T67383 WP_006247804.1 NZ_CP017548:1961576-1961845 [Mannheimia haemolytica]
MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKYRDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVR
LGSHAELFG

Download         Length: 270 bp

>T67383 NZ_CP017548:1961576-1961845 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9913.38 Da        Isoelectric Point: 7.0082

>AT67383 WP_006247803.1 NZ_CP017548:1961306-1961572 [Mannheimia haemolytica]
MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAKTKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEA
FHKAMLAE

Download         Length: 267 bp

>AT67383 NZ_CP017548:1961306-1961572 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZYA9

Antitoxin

Source ID Structure
AlphaFold DB A0A547E920

References