Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1179444..1180092 Replicon chromosome
Accession NZ_CP017548
Organism Mannheimia haemolytica strain 1319

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag BG580_RS06185 Protein ID WP_006251221.1
Coordinates 1179916..1180092 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag BG580_RS06180 Protein ID WP_006251222.1
Coordinates 1179444..1179860 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG580_RS06140 1175746..1176180 + 435 WP_006251230.1 hypothetical protein -
BG580_RS06145 1176195..1177184 + 990 WP_006251229.1 encapsulin -
BG580_RS06150 1177196..1177447 + 252 WP_015484272.1 hypothetical protein -
BG580_RS06155 1177450..1177827 + 378 WP_006251227.1 hypothetical protein -
BG580_RS06160 1177827..1178171 + 345 WP_006251226.1 hypothetical protein -
BG580_RS06165 1178173..1178541 + 369 WP_006251225.1 hypothetical protein -
BG580_RS06170 1178538..1178918 + 381 WP_006251224.1 hypothetical protein -
BG580_RS06175 1178922..1179404 + 483 WP_006251223.1 phage tail protein -
BG580_RS06180 1179444..1179860 - 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG580_RS06185 1179916..1180092 - 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
BG580_RS06190 1180188..1180859 + 672 WP_006251220.1 hypothetical protein -
BG580_RS06195 1180874..1181107 - 234 WP_020824316.1 hypothetical protein -
BG580_RS06200 1181276..1181734 + 459 WP_006251218.1 hypothetical protein -
BG580_RS06210 1182279..1184774 + 2496 WP_006251216.1 phage tail length tape measure family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1073597..1197182 123585


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T67382 WP_006251221.1 NZ_CP017548:c1180092-1179916 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T67382 NZ_CP017548:c1180092-1179916 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT67382 WP_006251222.1 NZ_CP017548:c1179860-1179444 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT67382 NZ_CP017548:c1179860-1179444 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References