Detailed information of TA system TA067381

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1131146..1131786
• Replicon: chromosome
• Accession: NZ_CP017548
• Organism: Mannheimia haemolytica strain 1319

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A378NFX7
• Locus tag: BG580_RS05790
• Protein ID: WP_006250075.1
• Coordinates: 1131604..1131786 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378NFW9
• Locus tag: BG580_RS05785
• Protein ID: WP_006250074.1
• Coordinates: 1131146..1131565 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG580_RS05760	1126693..1127499	+	807	WP_006248821.1	tryptophan synthase subunit alpha	-
BG580_RS05765	1127549..1128955	+	1407	WP_006253513.1	YdgA family protein	-
BG580_RS05770	1129022..1130028	-	1007	Protein_1123	IS481 family transposase	-
BG580_RS05780	1130166..1130810	-	645	WP_006253274.1	tyrosine-type recombinase/integrase	-
BG580_RS05785	1131146..1131565	-	420	WP_006250074.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG580_RS05790	1131604..1131786	-	183	WP_006250075.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG580_RS05795	1131918..1132403	-	486	WP_015484250.1	hypothetical protein	-
BG580_RS05800	1132408..1132788	-	381	WP_006248823.1	hypothetical protein	-
BG580_RS05805	1132863..1133546	-	684	WP_006248824.1	helix-turn-helix transcriptional regulator	-
BG580_RS05810	1133677..1133877	+	201	WP_006248825.1	helix-turn-helix domain-containing protein	-
BG580_RS14575	1133898..1134965	+	1068	WP_006253284.1	hypothetical protein	-
BG580_RS14580	1134975..1135133	+	159	WP_006247809.1	hypothetical protein	-
BG580_RS05825	1135193..1136233	-	1041	WP_015587043.1	IS481 family transposase	-
BG580_RS05835	1136350..1136649	-	300	WP_006247810.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1073597..1197182	123585
	-	flank	IS/Tn	-	-	1135193..1136233	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-96)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.03 Da
• Isoelectric Point: 10.3446

>T67381 WP_006250075.1 NZ_CP017548:c1131786-1131604 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T67381 NZ_CP017548:c1131786-1131604 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15938.30 Da
• Isoelectric Point: 4.6963

>AT67381 WP_006250074.1 NZ_CP017548:c1131565-1131146 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Nucleotide

• Length: 420 bp

>AT67381 NZ_CP017548:c1131565-1131146 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A378NFX7

Antitoxin

• AlphaFold DB: A0A378NFW9