Detailed information of TA system TA067376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2423376..2424024
• Replicon: chromosome
• Accession: NZ_CP017547
• Organism: Mannheimia haemolytica strain 1329

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A249A099
• Locus tag: BG543_RS12375
• Protein ID: WP_006251221.1
• Coordinates: 2423376..2423552 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A248ZZL6
• Locus tag: BG543_RS12380
• Protein ID: WP_006251222.1
• Coordinates: 2423608..2424024 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG543_RS12350	2418688..2421183	-	2496	WP_061888644.1	phage tail length tape measure family protein	-
BG543_RS12355	2421270..2421665	-	396	WP_006251217.1	hypothetical protein	-
BG543_RS12360	2421733..2422191	-	459	WP_006251218.1	hypothetical protein	-
BG543_RS12365	2422360..2422593	+	234	WP_020824316.1	hypothetical protein	-
BG543_RS12370	2422608..2423279	-	672	WP_006251220.1	hypothetical protein	-
BG543_RS12375	2423376..2423552	+	177	WP_006251221.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG543_RS12380	2423608..2424024	+	417	WP_006251222.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG543_RS12385	2424064..2424546	-	483	WP_006251223.1	phage tail protein	-
BG543_RS12390	2424550..2424930	-	381	WP_006251224.1	hypothetical protein	-
BG543_RS12395	2424927..2425295	-	369	WP_006251225.1	hypothetical protein	-
BG543_RS12400	2425297..2425641	-	345	WP_006251226.1	hypothetical protein	-
BG543_RS12405	2425641..2426018	-	378	WP_006251227.1	hypothetical protein	-
BG543_RS12410	2426021..2426245	-	225	WP_021265457.1	hypothetical protein	-
BG543_RS12415	2426257..2427243	-	987	WP_062627922.1	encapsulin	-
BG543_RS12420	2427258..2427692	-	435	WP_006251230.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2409702..2461308	51606

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.79 Da
• Isoelectric Point: 10.7708

>T67376 WP_006251221.1 NZ_CP017547:2423376-2423552 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T67376 NZ_CP017547:2423376-2423552 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15519.91 Da
• Isoelectric Point: 4.3508

>AT67376 WP_006251222.1 NZ_CP017547:2423608-2424024 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Nucleotide

• Length: 417 bp

>AT67376 NZ_CP017547:2423608-2424024 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Toxin

• AlphaFold DB: A0A249A099

Antitoxin

• AlphaFold DB: A0A248ZZL6