67375

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-RelB
Location 2269467..2269989 Replicon chromosome
Accession NZ_CP017547
Organism Mannheimia haemolytica strain 1329

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZX63
Locus tag BG543_RS11530 Protein ID WP_006250322.1
Coordinates 2269467..2269736 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A248ZY54
Locus tag BG543_RS11535 Protein ID WP_006250323.1
Coordinates 2269723..2269989 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG543_RS11510 2264787..2265221 - 435 WP_006250318.1 D-tyrosyl-tRNA(Tyr) deacylase -
BG543_RS11515 2265196..2266041 - 846 WP_006251985.1 virulence factor BrkB family protein -
BG543_RS11520 2266043..2267281 - 1239 WP_006251984.1 glutamate-5-semialdehyde dehydrogenase -
BG543_RS11525 2267565..2269448 + 1884 WP_006251983.1 DNA mismatch repair endonuclease MutL -
BG543_RS11530 2269467..2269736 - 270 WP_006250322.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
BG543_RS11535 2269723..2269989 - 267 WP_006250323.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BG543_RS11540 2270435..2271388 + 954 WP_006251982.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
BG543_RS11545 2271457..2271729 + 273 WP_006250326.1 RNA chaperone Hfq -
BG543_RS11550 2271749..2273134 + 1386 WP_020824252.1 GTPase HflX -
BG543_RS11560 2273471..2274511 + 1041 WP_020824044.1 IS481 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2273471..2274511 1040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-84)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10447.99 Da        Isoelectric Point: 6.4732

>T67375 WP_006250322.1 NZ_CP017547:c2269736-2269467 [Mannheimia haemolytica]
MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYCDHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLR
IGSHSHLFG

Download         Length: 270 bp

>T67375 NZ_CP017547:c2269736-2269467 [Mannheimia haemolytica]
ATGCGAGAGATTGAGTATAGTAATGCTTTTAAACGTGACTATAAAAAATGTGCGGTTGAGTCTTTATCCTCTTCATTAAT
TGAGGCGTTGTATTTTCTGATTAATGACAAGCCATTACCTGAGAAATACTGCGATCACGCTTTAACCGGCGAATGGAAAG
GTTTTAGGGATTGCCATATCCAACCTGATTTGGTTCTTATCTATCGAAAAGTGGAAGATGATAAGCTAGAGCTGCTGCGA
ATTGGCAGCCACTCTCATTTATTTGGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9738.10 Da        Isoelectric Point: 4.7954

>AT67375 WP_006250323.1 NZ_CP017547:c2269989-2269723 [Mannheimia haemolytica]
MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATEKRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADL
MEQLNARD

Download         Length: 267 bp

>AT67375 NZ_CP017547:c2269989-2269723 [Mannheimia haemolytica]
ATGGCAAATAGTGCATTAGTAAGCGTAAGAATCAGCCCTGAAATTAAAGAGCAAGCAAGTGAGGTGTTAGCCGGTATCGG
CTTGACTGTCTCTGATGTGATGCGAATGACATTAGCGAAAATCGCAACAGAAAAACGTTTTCAGTTTGATTATCAGCCTA
ATTTTGAGACAGCAGAAGTATTAAGGGCTGTTAAGAATGGCGAGGAAAAGCTACATTCAGCAAATAGTATTGCGGATTTA
ATGGAGCAACTCAATGCGAGAGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZX63

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZY54

References