Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 2082661..2083301 Replicon chromosome
Accession NZ_CP017547
Organism Mannheimia haemolytica strain 1329

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG543_RS10505 Protein ID WP_006250075.1
Coordinates 2082661..2082843 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NET2
Locus tag BG543_RS10510 Protein ID WP_006250984.1
Coordinates 2082882..2083301 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG543_RS10470 2078232..2079278 - 1047 WP_020824125.1 conserved phage C-terminal domain-containing protein -
BG543_RS10475 2079275..2080114 - 840 WP_006252350.1 Rha family transcriptional regulator -
BG543_RS10480 2080290..2080550 - 261 WP_006252483.1 hypothetical protein -
BG543_RS10485 2080571..2080771 - 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG543_RS10490 2080902..2081585 + 684 WP_006252485.1 helix-turn-helix transcriptional regulator -
BG543_RS10495 2081660..2082040 + 381 WP_006248823.1 hypothetical protein -
BG543_RS10500 2082045..2082530 + 486 WP_006252741.1 hypothetical protein -
BG543_RS10505 2082661..2082843 + 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG543_RS10510 2082882..2083301 + 420 WP_006250984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG543_RS10515 2083368..2083673 - 306 WP_006250985.1 HigA family addiction module antidote protein -
BG543_RS10520 2083682..2083957 - 276 WP_006250986.1 type II toxin-antitoxin system RelE/ParE family toxin -
BG543_RS10525 2084247..2084477 + 231 WP_006250987.1 hypothetical protein -
BG543_RS10530 2084956..2085279 - 324 WP_006250988.1 hypothetical protein -
BG543_RS10535 2085472..2085657 + 186 WP_020824222.1 hypothetical protein -
BG543_RS14175 2085641..2085805 + 165 WP_020824223.1 hypothetical protein -
BG543_RS10540 2085811..2086269 + 459 WP_006253113.1 single-stranded DNA-binding protein -
BG543_RS10545 2086305..2086664 + 360 WP_006250991.1 hypothetical protein -
BG543_RS10550 2086736..2087539 + 804 WP_020824224.1 DUF2303 family protein -
BG543_RS10555 2087633..2088067 + 435 WP_020824225.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2049257..2093152 43895


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T67374 WP_006250075.1 NZ_CP017547:2082661-2082843 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T67374 NZ_CP017547:2082661-2082843 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15957.34 Da        Isoelectric Point: 4.6983

>AT67374 WP_006250984.1 NZ_CP017547:2082882-2083301 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH

Download         Length: 420 bp

>AT67374 NZ_CP017547:2082882-2083301 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATACCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGTAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACGCT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NET2

References