Detailed information of TA system TA067359

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1046177..1046825
• Replicon: chromosome
• Accession: NZ_CP017545
• Organism: Mannheimia haemolytica strain 1367

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A249A099
• Locus tag: BG558_RS05400
• Protein ID: WP_006251221.1
• Coordinates: 1046649..1046825 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A248ZZL6
• Locus tag: BG558_RS05395
• Protein ID: WP_006251222.1
• Coordinates: 1046177..1046593 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG558_RS05355	1042470..1042904	+	435	WP_006251230.1	hypothetical protein	-
BG558_RS05360	1042919..1043908	+	990	WP_006251229.1	encapsulin	-
BG558_RS05365	1043920..1044180	+	261	WP_020824317.1	hypothetical protein	-
BG558_RS05370	1044183..1044560	+	378	WP_006251227.1	hypothetical protein	-
BG558_RS05375	1044560..1044904	+	345	WP_006251226.1	hypothetical protein	-
BG558_RS05380	1044906..1045274	+	369	WP_006251225.1	hypothetical protein	-
BG558_RS05385	1045271..1045651	+	381	WP_006251224.1	hypothetical protein	-
BG558_RS05390	1045655..1046137	+	483	WP_006251223.1	phage tail protein	-
BG558_RS05395	1046177..1046593	-	417	WP_006251222.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG558_RS05400	1046649..1046825	-	177	WP_006251221.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG558_RS05405	1046921..1047592	+	672	WP_006251220.1	hypothetical protein	-
BG558_RS05410	1047607..1047876	-	270	WP_006251219.1	hypothetical protein	-
BG558_RS05415	1048009..1048467	+	459	WP_006251218.1	hypothetical protein	-
BG558_RS05420	1048535..1048930	+	396	WP_006251217.1	hypothetical protein	-
BG558_RS05425	1049017..1051512	+	2496	WP_061888644.1	phage tail length tape measure family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	lgtF / waaQ	1013460..1069727	56267

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.79 Da
• Isoelectric Point: 10.7708

>T67359 WP_006251221.1 NZ_CP017545:c1046825-1046649 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T67359 NZ_CP017545:c1046825-1046649 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15519.91 Da
• Isoelectric Point: 4.3508

>AT67359 WP_006251222.1 NZ_CP017545:c1046593-1046177 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Nucleotide

• Length: 417 bp

>AT67359 NZ_CP017545:c1046593-1046177 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Toxin

• AlphaFold DB: A0A249A099

Antitoxin

• AlphaFold DB: A0A248ZZL6