Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 1953988..1954527 Replicon chromosome
Accession NZ_CP017544
Organism Mannheimia haemolytica strain 1373

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZYA9
Locus tag BG581_RS10270 Protein ID WP_006247804.1
Coordinates 1954258..1954527 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A547E920
Locus tag BG581_RS10265 Protein ID WP_006247803.1
Coordinates 1953988..1954254 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG581_RS10210 1949388..1950023 - 636 Protein_1990 transposase -
BG581_RS10220 1950124..1950834 - 711 Protein_1991 tyrosine-type recombinase/integrase -
BG581_RS10225 1950738..1951061 - 324 WP_006253316.1 helix-turn-helix domain-containing protein -
BG581_RS10230 1951142..1951309 - 168 WP_006253314.1 DNA cytosine methyltransferase -
BG581_RS10235 1951317..1951691 - 375 WP_006247798.1 hypothetical protein -
BG581_RS10240 1951841..1952068 + 228 Protein_1995 phage terminase large subunit family protein -
BG581_RS10245 1952093..1952488 + 396 WP_006247799.1 phage tail protein -
BG581_RS10250 1952520..1953161 + 642 WP_006247800.1 hypothetical protein -
BG581_RS10255 1953244..1953645 + 402 WP_006247801.1 hypothetical protein -
BG581_RS10260 1953690..1953920 + 231 WP_006247802.1 DUF4035 domain-containing protein -
BG581_RS10265 1953988..1954254 + 267 WP_006247803.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BG581_RS10270 1954258..1954527 + 270 WP_006247804.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
BG581_RS10275 1954602..1954865 + 264 WP_006247805.1 hypothetical protein -
BG581_RS10280 1954925..1955635 + 711 WP_006253312.1 tape measure protein -
BG581_RS10285 1955613..1955903 + 291 Protein_2004 C40 family peptidase -
BG581_RS10290 1955893..1956612 + 720 WP_006247806.1 preprotein translocase subunit YajC -
BG581_RS10295 1956834..1957460 + 627 WP_006247807.1 tail assembly protein -
BG581_RS10300 1957509..1958120 + 612 WP_006253310.1 hypothetical protein -
BG581_RS10305 1958231..1958917 + 687 WP_006247808.1 hypothetical protein -
BG581_RS14670 1958927..1959085 + 159 WP_006247809.1 hypothetical protein -
BG581_RS10310 1959094..1959393 - 300 WP_006247810.1 XRE family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1941375..1965581 24206
- flank IS/Tn - - 1949331..1950023 692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-75)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10278.90 Da        Isoelectric Point: 6.2121

>T67353 WP_006247804.1 NZ_CP017544:1954258-1954527 [Mannheimia haemolytica]
MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKYRDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVR
LGSHAELFG

Download         Length: 270 bp

>T67353 NZ_CP017544:1954258-1954527 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9913.38 Da        Isoelectric Point: 7.0082

>AT67353 WP_006247803.1 NZ_CP017544:1953988-1954254 [Mannheimia haemolytica]
MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAKTKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEA
FHKAMLAE

Download         Length: 267 bp

>AT67353 NZ_CP017544:1953988-1954254 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZYA9

Antitoxin

Source ID Structure
AlphaFold DB A0A547E920

References