Detailed information of TA system TA067351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1131037..1131677
• Replicon: chromosome
• Accession: NZ_CP017544
• Organism: Mannheimia haemolytica strain 1373

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A378NFX7
• Locus tag: BG581_RS05775
• Protein ID: WP_006250075.1
• Coordinates: 1131495..1131677 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378NFW9
• Locus tag: BG581_RS05770
• Protein ID: WP_006250074.1
• Coordinates: 1131037..1131456 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG581_RS05745	1126584..1127390	+	807	WP_006248821.1	tryptophan synthase subunit alpha	-
BG581_RS05750	1127440..1128846	+	1407	WP_006253513.1	YdgA family protein	-
BG581_RS05755	1128913..1129919	-	1007	Protein_1122	IS481 family transposase	-
BG581_RS05765	1130057..1130701	-	645	WP_006253274.1	tyrosine-type recombinase/integrase	-
BG581_RS05770	1131037..1131456	-	420	WP_006250074.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG581_RS05775	1131495..1131677	-	183	WP_006250075.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG581_RS05780	1131809..1132294	-	486	WP_015484250.1	hypothetical protein	-
BG581_RS05785	1132299..1132679	-	381	WP_006248823.1	hypothetical protein	-
BG581_RS05790	1132754..1133437	-	684	WP_006248824.1	helix-turn-helix transcriptional regulator	-
BG581_RS05795	1133568..1133768	+	201	WP_006248825.1	helix-turn-helix domain-containing protein	-
BG581_RS14530	1133789..1134856	+	1068	WP_006253284.1	hypothetical protein	-
BG581_RS14535	1134866..1135024	+	159	WP_006247809.1	hypothetical protein	-
BG581_RS05810	1135084..1136124	-	1041	WP_015587043.1	IS481 family transposase	-
BG581_RS05820	1136241..1136540	-	300	WP_006247810.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1073488..1197224	123736
	-	flank	IS/Tn	-	-	1135084..1136124	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-96)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.03 Da
• Isoelectric Point: 10.3446

>T67351 WP_006250075.1 NZ_CP017544:c1131677-1131495 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T67351 NZ_CP017544:c1131677-1131495 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15938.30 Da
• Isoelectric Point: 4.6963

>AT67351 WP_006250074.1 NZ_CP017544:c1131456-1131037 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Nucleotide

• Length: 420 bp

>AT67351 NZ_CP017544:c1131456-1131037 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A378NFX7

Antitoxin

• AlphaFold DB: A0A378NFW9