Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2448691..2449280 Replicon chromosome
Accession NZ_CP017543
Organism Mannheimia haemolytica strain 1383

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag BG611_RS12625 Protein ID WP_006248516.1
Coordinates 2448948..2449280 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag BG611_RS12620 Protein ID WP_006248517.1
Coordinates 2448691..2448951 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG611_RS12595 2443870..2445015 - 1146 WP_006248526.1 FMN-dependent L-lactate dehydrogenase LldD -
BG611_RS12600 2445627..2446271 + 645 WP_006248521.1 RNA pyrophosphohydrolase -
BG611_RS12605 2446273..2447061 + 789 WP_006248520.1 sulfite exporter TauE/SafE family protein -
BG611_RS12610 2447069..2447866 + 798 WP_006248519.1 prolipoprotein diacylglyceryl transferase -
BG611_RS12615 2447953..2448621 + 669 WP_006248518.1 phosphoglycolate phosphatase -
BG611_RS12620 2448691..2448951 + 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BG611_RS12625 2448948..2449280 + 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BG611_RS12630 2449407..2450438 + 1032 WP_006248514.1 tryptophan--tRNA ligase -
BG611_RS12635 2450502..2451257 - 756 WP_006248513.1 M48 family metallopeptidase -
BG611_RS12640 2451313..2451840 - 528 WP_006248512.1 inorganic diphosphatase -
BG611_RS12660 2452420..2453454 + 1035 WP_006248509.1 endolytic transglycosylase MltG -
BG611_RS12665 2453465..2454085 + 621 WP_006248508.1 dTMP kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T67347 WP_006248516.1 NZ_CP017543:2448948-2449280 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

>T67347 NZ_CP017543:2448948-2449280 [Mannheimia haemolytica]
ATGAAACGTGGCGATATTTACTGGCTCGATCTTGATCCAACCTTAGGACACGAACAATCAGGCTTCCGCCCCGTTGTAAT
TGTTTCCGCAACTGCATTTAATCTAGCCAGTAAACTGCCTGTGGTTGTGCCGATTACTAACGGTGGAGCTTTTGCTGAAA
GACTGGGCTTTGCCGTTTCATTAAAAGATGCCGGCTTAAAAACAACAGGGATTATTCGATGCGATCAACCTCGCACAGTC
GATATTGCACAACGCCATGGAAAATTTATCGAAGCCTTACCCGAAAGCCTACTTGATGAAATTATGGCAAGGGTTTCAGT
GATTTTTGAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT67347 WP_006248517.1 NZ_CP017543:2448691-2448951 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp

>AT67347 NZ_CP017543:2448691-2448951 [Mannheimia haemolytica]
ATGCCAACAATCGCATTACGTCAGGTCGGTGGCTCGGTTGCAATGGTGATTCCGCCTGCTTTTTTAGAGGCACTCAAGCT
CTCCGTCGGTAGCAAAGTGGATTTGTCTTTAAACGACAACCAACTCGTGATTAATCCTGCCCGCCCAAAAGTGAAATACA
GCTTAGAGGAATTATTAAGACAATCCGATTTTGAGGCAGCAAGGAATGAAGAAAGCCAATCGTGGTTATCCGAGCCGGCA
ATAGGAAAAGAAATTTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZY19

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References