Detailed information of TA system TA067336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1655495..1656135
• Replicon: chromosome
• Accession: NZ_CP017542
• Organism: Mannheimia haemolytica strain 1405

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A378NFX7
• Locus tag: BG593_RS08575
• Protein ID: WP_006250075.1
• Coordinates: 1655495..1655677 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378NFW9
• Locus tag: BG593_RS08580
• Protein ID: WP_006250074.1
• Coordinates: 1655716..1656135 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG593_RS08530	1651042..1651335	-	294	WP_006247812.1	hypothetical protein	-
BG593_RS08535	1651479..1651868	+	390	WP_015484247.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BG593_RS08540	1651840..1652139	+	300	WP_006247810.1	XRE family transcriptional regulator	-
BG593_RS14135	1652148..1652306	-	159	WP_006247809.1	hypothetical protein	-
BG593_RS14140	1652316..1653383	-	1068	WP_006253284.1	hypothetical protein	-
BG593_RS08555	1653404..1653604	-	201	WP_006248825.1	helix-turn-helix domain-containing protein	-
BG593_RS08560	1653735..1654418	+	684	WP_006248824.1	helix-turn-helix transcriptional regulator	-
BG593_RS08565	1654493..1654873	+	381	WP_006248823.1	hypothetical protein	-
BG593_RS08570	1654878..1655363	+	486	WP_015484250.1	hypothetical protein	-
BG593_RS08575	1655495..1655677	+	183	WP_006250075.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG593_RS08580	1655716..1656135	+	420	WP_006250074.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG593_RS08585	1656471..1657115	+	645	WP_006253274.1	tyrosine-type recombinase/integrase	-
BG593_RS08595	1657253..1658293	+	1041	WP_020910232.1	IS481 family transposase	-
BG593_RS08600	1658360..1659766	-	1407	WP_006253513.1	YdgA family protein	-
BG593_RS08605	1659816..1660622	-	807	WP_006248821.1	tryptophan synthase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1624265..1714133	89868
	-	flank	IS/Tn	-	-	1657253..1658293	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-96)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.03 Da
• Isoelectric Point: 10.3446

>T67336 WP_006250075.1 NZ_CP017542:1655495-1655677 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T67336 NZ_CP017542:1655495-1655677 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15938.30 Da
• Isoelectric Point: 4.6963

>AT67336 WP_006250074.1 NZ_CP017542:1655716-1656135 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Nucleotide

• Length: 420 bp

>AT67336 NZ_CP017542:1655716-1656135 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A378NFX7

Antitoxin

• AlphaFold DB: A0A378NFW9