Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2460908..2461497 Replicon chromosome
Accession NZ_CP017541
Organism Mannheimia haemolytica strain 1450

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag BG588_RS12750 Protein ID WP_006248516.1
Coordinates 2461165..2461497 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag BG588_RS12745 Protein ID WP_006248517.1
Coordinates 2460908..2461168 (+) Length 87 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG588_RS12720 2456087..2457232 - 1146 WP_006248526.1 FMN-dependent L-lactate dehydrogenase LldD -
BG588_RS12725 2457844..2458488 + 645 WP_006248521.1 RNA pyrophosphohydrolase -
BG588_RS12730 2458490..2459278 + 789 WP_006248520.1 sulfite exporter TauE/SafE family protein -
BG588_RS12735 2459286..2460083 + 798 WP_006248519.1 prolipoprotein diacylglyceryl transferase -
BG588_RS12740 2460170..2460838 + 669 WP_006248518.1 phosphoglycolate phosphatase -
BG588_RS12745 2460908..2461168 + 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BG588_RS12750 2461165..2461497 + 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BG588_RS12755 2461624..2462655 + 1032 WP_006248514.1 tryptophan--tRNA ligase -
BG588_RS12760 2462719..2463474 - 756 WP_006248513.1 M48 family metallopeptidase -
BG588_RS12765 2463530..2464057 - 528 WP_006248512.1 inorganic diphosphatase -
BG588_RS12785 2464637..2465671 + 1035 WP_006248509.1 endolytic transglycosylase MltG -
BG588_RS12790 2465682..2466302 + 621 WP_006248508.1 dTMP kinase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-109)

Antitoxin

(7-48)


Sequences


Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T67331 WP_006248516.1 NZ_CP017541:2461165-2461497 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

>T67331 NZ_CP017541:2461165-2461497 [Mannheimia haemolytica]
ATGAAACGTGGCGATATTTACTGGCTCGATCTTGATCCAACCTTAGGACACGAACAATCAGGCTTCCGCCCCGTTGTAAT
TGTTTCCGCAACTGCATTTAATCTAGCCAGTAAACTGCCTGTGGTTGTGCCGATTACTAACGGTGGAGCTTTTGCTGAAA
GACTGGGCTTTGCCGTTTCATTAAAAGATGCCGGCTTAAAAACAACAGGGATTATTCGATGCGATCAACCTCGCACAGTC
GATATTGCACAACGCCATGGAAAATTTATCGAAGCCTTACCCGAAAGCCTACTTGATGAAATTATGGCAAGGGTTTCAGT
GATTTTTGAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT67331 WP_006248517.1 NZ_CP017541:2460908-2461168 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp

>AT67331 NZ_CP017541:2460908-2461168 [Mannheimia haemolytica]
ATGCCAACAATCGCATTACGTCAGGTCGGTGGCTCGGTTGCAATGGTGATTCCGCCTGCTTTTTTAGAGGCACTCAAGCT
CTCCGTCGGTAGCAAAGTGGATTTGTCTTTAAACGACAACCAACTCGTGATTAATCCTGCCCGCCCAAAAGTGAAATACA
GCTTAGAGGAATTATTAAGACAATCCGATTTTGAGGCAGCAAGGAATGAAGAAAGCCAATCGTGGTTATCCGAGCCGGCA
ATAGGAAAAGAAATTTTATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A248ZY19


Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References