Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1707762..1708410 Replicon chromosome
Accession NZ_CP017541
Organism Mannheimia haemolytica strain 1450

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag BG588_RS08945 Protein ID WP_006251221.1
Coordinates 1708234..1708410 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag BG588_RS08940 Protein ID WP_006251222.1
Coordinates 1707762..1708178 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG588_RS08900 1704046..1704480 + 435 WP_006251230.1 hypothetical protein -
BG588_RS08905 1704495..1705484 + 990 WP_006251229.1 encapsulin -
BG588_RS14200 1705496..1705765 + 270 WP_020849873.1 hypothetical protein -
BG588_RS08915 1705768..1706145 + 378 WP_006251227.1 hypothetical protein -
BG588_RS08920 1706145..1706489 + 345 WP_006251226.1 hypothetical protein -
BG588_RS08925 1706491..1706859 + 369 WP_006251225.1 hypothetical protein -
BG588_RS08930 1706856..1707236 + 381 WP_006251224.1 hypothetical protein -
BG588_RS08935 1707240..1707722 + 483 WP_006251223.1 phage tail protein -
BG588_RS08940 1707762..1708178 - 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG588_RS08945 1708234..1708410 - 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
BG588_RS08950 1708506..1709177 + 672 WP_006251220.1 hypothetical protein -
BG588_RS08955 1709192..1709425 - 234 WP_020824316.1 hypothetical protein -
BG588_RS08960 1709594..1710052 + 459 WP_006251218.1 hypothetical protein -
BG588_RS08970 1710597..1713092 + 2496 WP_006251216.1 phage tail length tape measure family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1595577..1723732 128155


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T67328 WP_006251221.1 NZ_CP017541:c1708410-1708234 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T67328 NZ_CP017541:c1708410-1708234 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT67328 WP_006251222.1 NZ_CP017541:c1708178-1707762 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT67328 NZ_CP017541:c1708178-1707762 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References