Detailed information of TA system TA067327

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1655342..1655982
• Replicon: chromosome
• Accession: NZ_CP017541
• Organism: Mannheimia haemolytica strain 1450

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A378NFX7
• Locus tag: BG588_RS08525
• Protein ID: WP_006250075.1
• Coordinates: 1655800..1655982 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378NFW9
• Locus tag: BG588_RS08520
• Protein ID: WP_006250074.1
• Coordinates: 1655342..1655761 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG588_RS08495	1650855..1651661	+	807	WP_006248821.1	tryptophan synthase subunit alpha	-
BG588_RS08500	1651711..1653117	+	1407	WP_006253513.1	YdgA family protein	-
BG588_RS08505	1653184..1654224	-	1041	WP_015587044.1	IS481 family transposase	-
BG588_RS08515	1654362..1655006	-	645	WP_006253274.1	tyrosine-type recombinase/integrase	-
BG588_RS08520	1655342..1655761	-	420	WP_006250074.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG588_RS08525	1655800..1655982	-	183	WP_006250075.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG588_RS08530	1656114..1656599	-	486	WP_015484250.1	hypothetical protein	-
BG588_RS08535	1656604..1656984	-	381	WP_006248823.1	hypothetical protein	-
BG588_RS08540	1657059..1657742	-	684	WP_006248824.1	helix-turn-helix transcriptional regulator	-
BG588_RS08545	1657873..1658073	+	201	WP_006248825.1	helix-turn-helix domain-containing protein	-
BG588_RS14160	1658094..1659161	+	1068	WP_006253284.1	hypothetical protein	-
BG588_RS14165	1659171..1659329	+	159	WP_006247809.1	hypothetical protein	-
BG588_RS08560	1659338..1659637	-	300	WP_006247810.1	XRE family transcriptional regulator	-
BG588_RS08565	1659609..1659998	-	390	WP_015484247.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BG588_RS08570	1660142..1660435	+	294	WP_006247812.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1595577..1723732	128155
	-	flank	IS/Tn	-	-	1653184..1654224	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-96)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.03 Da
• Isoelectric Point: 10.3446

>T67327 WP_006250075.1 NZ_CP017541:c1655982-1655800 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T67327 NZ_CP017541:c1655982-1655800 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15938.30 Da
• Isoelectric Point: 4.6963

>AT67327 WP_006250074.1 NZ_CP017541:c1655761-1655342 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Nucleotide

• Length: 420 bp

>AT67327 NZ_CP017541:c1655761-1655342 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A378NFX7

Antitoxin

• AlphaFold DB: A0A378NFW9