Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 1814613..1815152 Replicon chromosome
Accession NZ_CP017540
Organism Mannheimia haemolytica strain 1451

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZYA9
Locus tag BG578_RS09360 Protein ID WP_006247804.1
Coordinates 1814883..1815152 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A547E920
Locus tag BG578_RS09355 Protein ID WP_006247803.1
Coordinates 1814613..1814879 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG578_RS09300 1810013..1810648 - 636 Protein_1811 transposase -
BG578_RS09310 1810749..1811459 - 711 Protein_1812 tyrosine-type recombinase/integrase -
BG578_RS09315 1811363..1811686 - 324 WP_006253316.1 helix-turn-helix domain-containing protein -
BG578_RS09320 1811767..1811934 - 168 WP_006253314.1 DNA cytosine methyltransferase -
BG578_RS09325 1811942..1812316 - 375 WP_006247798.1 hypothetical protein -
BG578_RS09330 1812466..1812693 + 228 Protein_1816 phage terminase large subunit family protein -
BG578_RS09335 1812718..1813113 + 396 WP_006247799.1 phage tail protein -
BG578_RS09340 1813145..1813786 + 642 WP_006247800.1 hypothetical protein -
BG578_RS09345 1813869..1814270 + 402 WP_006247801.1 hypothetical protein -
BG578_RS09350 1814315..1814545 + 231 WP_006247802.1 DUF4035 domain-containing protein -
BG578_RS09355 1814613..1814879 + 267 WP_006247803.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BG578_RS09360 1814883..1815152 + 270 WP_006247804.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
BG578_RS09365 1815227..1815490 + 264 WP_006247805.1 hypothetical protein -
BG578_RS09370 1815550..1816260 + 711 WP_006253312.1 tape measure protein -
BG578_RS09375 1816238..1816528 + 291 Protein_1825 C40 family peptidase -
BG578_RS09380 1816518..1817237 + 720 WP_006247806.1 preprotein translocase subunit YajC -
BG578_RS09385 1817459..1818085 + 627 Protein_1827 tail assembly protein -
BG578_RS09390 1818134..1818745 + 612 WP_006253310.1 hypothetical protein -
BG578_RS09395 1818856..1819542 + 687 WP_006247808.1 hypothetical protein -
BG578_RS13705 1819552..1819710 + 159 WP_006247809.1 hypothetical protein -
BG578_RS09400 1819719..1820018 - 300 WP_006247810.1 XRE family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1802000..1826206 24206
- flank IS/Tn - - 1809956..1810648 692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-75)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10278.90 Da        Isoelectric Point: 6.2121

>T67321 WP_006247804.1 NZ_CP017540:1814883-1815152 [Mannheimia haemolytica]
MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKYRDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVR
LGSHAELFG

Download         Length: 270 bp

>T67321 NZ_CP017540:1814883-1815152 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9913.38 Da        Isoelectric Point: 7.0082

>AT67321 WP_006247803.1 NZ_CP017540:1814613-1814879 [Mannheimia haemolytica]
MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAKTKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEA
FHKAMLAE

Download         Length: 267 bp

>AT67321 NZ_CP017540:1814613-1814879 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZYA9

Antitoxin

Source ID Structure
AlphaFold DB A0A547E920

References