Detailed information of TA system TA067320

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1595924..1596564
• Replicon: chromosome
• Accession: NZ_CP017540
• Organism: Mannheimia haemolytica strain 1451

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A378NFX7
• Locus tag: BG578_RS08160
• Protein ID: WP_006250075.1
• Coordinates: 1595924..1596106 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378NFW9
• Locus tag: BG578_RS08165
• Protein ID: WP_006250074.1
• Coordinates: 1596145..1596564 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG578_RS08115	1591471..1591764	-	294	WP_006247812.1	hypothetical protein	-
BG578_RS08120	1591908..1592297	+	390	WP_015484247.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BG578_RS08125	1592269..1592568	+	300	WP_006247810.1	XRE family transcriptional regulator	-
BG578_RS13685	1592577..1592735	-	159	WP_006247809.1	hypothetical protein	-
BG578_RS13690	1592745..1593812	-	1068	WP_006253284.1	hypothetical protein	-
BG578_RS08140	1593833..1594033	-	201	WP_006248825.1	helix-turn-helix domain-containing protein	-
BG578_RS08145	1594164..1594847	+	684	WP_006248824.1	helix-turn-helix transcriptional regulator	-
BG578_RS08150	1594922..1595302	+	381	WP_006248823.1	hypothetical protein	-
BG578_RS08155	1595307..1595792	+	486	WP_015484250.1	hypothetical protein	-
BG578_RS08160	1595924..1596106	+	183	WP_006250075.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG578_RS08165	1596145..1596564	+	420	WP_006250074.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG578_RS08170	1596900..1597544	+	645	WP_006253274.1	tyrosine-type recombinase/integrase	-
BG578_RS08180	1597682..1598722	+	1041	WP_147010043.1	IS481 family transposase	-
BG578_RS08185	1598789..1600195	-	1407	WP_006253513.1	YdgA family protein	-
BG578_RS08190	1600245..1601051	-	807	WP_006248821.1	tryptophan synthase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1588328..1600195	11867
	-	flank	IS/Tn	-	-	1597682..1598722	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-96)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.03 Da
• Isoelectric Point: 10.3446

>T67320 WP_006250075.1 NZ_CP017540:1595924-1596106 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T67320 NZ_CP017540:1595924-1596106 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15938.30 Da
• Isoelectric Point: 4.6963

>AT67320 WP_006250074.1 NZ_CP017540:1596145-1596564 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Nucleotide

• Length: 420 bp

>AT67320 NZ_CP017540:1596145-1596564 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A378NFX7

Antitoxin

• AlphaFold DB: A0A378NFW9