Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1282667..1283315 Replicon chromosome
Accession NZ_CP017540
Organism Mannheimia haemolytica strain 1451

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag BG578_RS06640 Protein ID WP_006251221.1
Coordinates 1283139..1283315 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag BG578_RS06635 Protein ID WP_006251222.1
Coordinates 1282667..1283083 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG578_RS06595 1278951..1279385 + 435 WP_006251230.1 hypothetical protein -
BG578_RS06600 1279400..1280389 + 990 WP_006251229.1 encapsulin -
BG578_RS13660 1280401..1280670 + 270 WP_020849873.1 hypothetical protein -
BG578_RS06610 1280673..1281050 + 378 WP_006251227.1 hypothetical protein -
BG578_RS06615 1281050..1281394 + 345 WP_006251226.1 hypothetical protein -
BG578_RS06620 1281396..1281764 + 369 WP_006251225.1 hypothetical protein -
BG578_RS06625 1281761..1282141 + 381 WP_006251224.1 hypothetical protein -
BG578_RS06630 1282145..1282627 + 483 WP_006251223.1 phage tail protein -
BG578_RS06635 1282667..1283083 - 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG578_RS06640 1283139..1283315 - 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
BG578_RS06645 1283411..1284082 + 672 WP_006251220.1 hypothetical protein -
BG578_RS06650 1284097..1284330 - 234 WP_020824316.1 hypothetical protein -
BG578_RS06655 1284499..1284957 + 459 WP_006251218.1 hypothetical protein -
BG578_RS06665 1285502..1287997 + 2496 WP_006251216.1 phage tail length tape measure family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 1281208..1455375 174167


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T67319 WP_006251221.1 NZ_CP017540:c1283315-1283139 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T67319 NZ_CP017540:c1283315-1283139 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT67319 WP_006251222.1 NZ_CP017540:c1283083-1282667 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT67319 NZ_CP017540:c1283083-1282667 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References