Detailed information of TA system TA067309

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 300083..300731
• Replicon: chromosome
• Accession: NZ_CP017539
• Organism: Mannheimia haemolytica strain 1454

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A249A099
• Locus tag: BG603_RS01655
• Protein ID: WP_006251221.1
• Coordinates: 300555..300731 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A248ZZL6
• Locus tag: BG603_RS01650
• Protein ID: WP_006251222.1
• Coordinates: 300083..300499 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG603_RS01595	295208..295483	+	276	WP_006250986.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BG603_RS01600	295492..295797	+	306	WP_006253368.1	HigA family addiction module antidote protein	-
BG603_RS01605	295921..296961	-	1041	WP_015587044.1	IS481 family transposase	-
BG603_RS01610	296960..297061	+	102	Protein_316	DNA primase	-
BG603_RS01615	297062..297823	+	762	Protein_317	encapsulin	-
BG603_RS01620	297835..298086	+	252	WP_015484272.1	hypothetical protein	-
BG603_RS01630	298466..298810	+	345	WP_006251226.1	hypothetical protein	-
BG603_RS01635	298812..299180	+	369	WP_006251225.1	hypothetical protein	-
BG603_RS01640	299177..299557	+	381	WP_006251224.1	hypothetical protein	-
BG603_RS01645	299561..300043	+	483	WP_006251223.1	phage tail protein	-
BG603_RS01650	300083..300499	-	417	WP_006251222.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG603_RS01655	300555..300731	-	177	WP_006251221.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG603_RS01660	300827..301498	+	672	WP_006251220.1	hypothetical protein	-
BG603_RS01665	301513..301746	-	234	WP_020824316.1	hypothetical protein	-
BG603_RS01670	301915..302373	+	459	WP_006251218.1	hypothetical protein	-
BG603_RS01680	302918..305413	+	2496	WP_006251216.1	phage tail length tape measure family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	285205..317824	32619
	-	flank	IS/Tn	-	-	295921..296961	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6640.79 Da
• Isoelectric Point: 10.7708

>T67309 WP_006251221.1 NZ_CP017539:c300731-300555 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Nucleotide

• Length: 177 bp

>T67309 NZ_CP017539:c300731-300555 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15519.91 Da
• Isoelectric Point: 4.3508

>AT67309 WP_006251222.1 NZ_CP017539:c300499-300083 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Nucleotide

• Length: 417 bp

>AT67309 NZ_CP017539:c300499-300083 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Structures

Toxin

• AlphaFold DB: A0A249A099

Antitoxin

• AlphaFold DB: A0A248ZZL6