Detailed information of TA system TA067308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 2615260..2615900
• Replicon: chromosome
• Accession: NZ_CP017538
• Organism: Mannheimia haemolytica strain 1471

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A378NFX7
• Locus tag: BG586_RS13580
• Protein ID: WP_006250075.1
• Coordinates: 2615260..2615442 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378NFW9
• Locus tag: BG586_RS13585
• Protein ID: WP_006250074.1
• Coordinates: 2615481..2615900 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG586_RS13535	2610397..2610696	+	300	WP_006247810.1	XRE family transcriptional regulator	-
BG586_RS13545	2610813..2611853	+	1041	WP_015587043.1	IS481 family transposase	-
BG586_RS14955	2611913..2612071	-	159	WP_006247809.1	hypothetical protein	-
BG586_RS14960	2612081..2613148	-	1068	WP_006253284.1	hypothetical protein	-
BG586_RS13560	2613169..2613369	-	201	WP_006248825.1	helix-turn-helix domain-containing protein	-
BG586_RS13565	2613500..2614183	+	684	WP_006248824.1	helix-turn-helix transcriptional regulator	-
BG586_RS13570	2614258..2614638	+	381	WP_006248823.1	hypothetical protein	-
BG586_RS13575	2614643..2615128	+	486	WP_015484250.1	hypothetical protein	-
BG586_RS13580	2615260..2615442	+	183	WP_006250075.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG586_RS13585	2615481..2615900	+	420	WP_006250074.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG586_RS13590	2616236..2616880	+	645	WP_006253274.1	tyrosine-type recombinase/integrase	-
BG586_RS13600	2617018..2618024	+	1007	Protein_2614	IS481 family transposase	-
BG586_RS13605	2618091..2619497	-	1407	WP_006253513.1	YdgA family protein	-
BG586_RS13610	2619547..2620353	-	807	WP_006248821.1	tryptophan synthase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2605650..2629548	23898
	-	flank	IS/Tn	-	-	2610813..2611853	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-96)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.03 Da
• Isoelectric Point: 10.3446

>T67308 WP_006250075.1 NZ_CP017538:2615260-2615442 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T67308 NZ_CP017538:2615260-2615442 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15938.30 Da
• Isoelectric Point: 4.6963

>AT67308 WP_006250074.1 NZ_CP017538:2615481-2615900 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Nucleotide

• Length: 420 bp

>AT67308 NZ_CP017538:2615481-2615900 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A378NFX7

Antitoxin

• AlphaFold DB: A0A378NFW9