Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2250203..2250851 Replicon chromosome
Accession NZ_CP017538
Organism Mannheimia haemolytica strain 1471

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag BG586_RS11690 Protein ID WP_006251221.1
Coordinates 2250675..2250851 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag BG586_RS11685 Protein ID WP_006251222.1
Coordinates 2250203..2250619 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG586_RS11645 2246514..2246948 + 435 WP_006251230.1 hypothetical protein -
BG586_RS11650 2246963..2247952 + 990 WP_006251229.1 encapsulin -
BG586_RS11655 2247964..2248206 + 243 WP_061888482.1 hypothetical protein -
BG586_RS11660 2248209..2248586 + 378 WP_006251227.1 hypothetical protein -
BG586_RS11665 2248586..2248930 + 345 WP_006251226.1 hypothetical protein -
BG586_RS11670 2248932..2249300 + 369 WP_006251225.1 hypothetical protein -
BG586_RS11675 2249297..2249677 + 381 WP_006251224.1 hypothetical protein -
BG586_RS11680 2249681..2250163 + 483 WP_006251223.1 phage tail protein -
BG586_RS11685 2250203..2250619 - 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG586_RS11690 2250675..2250851 - 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
BG586_RS11695 2250947..2251618 + 672 WP_006251220.1 hypothetical protein -
BG586_RS11700 2251633..2251866 - 234 WP_020824316.1 hypothetical protein -
BG586_RS11705 2252035..2252493 + 459 WP_006251218.1 hypothetical protein -
BG586_RS11715 2253038..2255533 + 2496 WP_006251216.1 phage tail length tape measure family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2219021..2267526 48505


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T67307 WP_006251221.1 NZ_CP017538:c2250851-2250675 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T67307 NZ_CP017538:c2250851-2250675 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT67307 WP_006251222.1 NZ_CP017538:c2250619-2250203 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT67307 NZ_CP017538:c2250619-2250203 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References