Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 1785330..1785869 Replicon chromosome
Accession NZ_CP017538
Organism Mannheimia haemolytica strain 1471

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZYA9
Locus tag BG586_RS09065 Protein ID WP_006247804.1
Coordinates 1785330..1785599 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A547E920
Locus tag BG586_RS09070 Protein ID WP_006247803.1
Coordinates 1785603..1785869 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG586_RS09025 1780464..1780763 + 300 WP_006247810.1 XRE family transcriptional regulator -
BG586_RS14810 1780772..1780930 - 159 WP_006247809.1 hypothetical protein -
BG586_RS09030 1780940..1781626 - 687 WP_006247808.1 hypothetical protein -
BG586_RS09035 1781737..1782348 - 612 WP_006253310.1 hypothetical protein -
BG586_RS09040 1782397..1783023 - 627 WP_006247807.1 tail assembly protein -
BG586_RS09045 1783245..1783964 - 720 WP_006247806.1 preprotein translocase subunit YajC -
BG586_RS09050 1783954..1784244 - 291 Protein_1724 C40 family peptidase -
BG586_RS09055 1784222..1784932 - 711 WP_006253312.1 tape measure protein -
BG586_RS09060 1784992..1785255 - 264 WP_006247805.1 hypothetical protein -
BG586_RS09065 1785330..1785599 - 270 WP_006247804.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
BG586_RS09070 1785603..1785869 - 267 WP_006247803.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BG586_RS09075 1785937..1786167 - 231 WP_006247802.1 DUF4035 domain-containing protein -
BG586_RS09080 1786212..1786613 - 402 WP_006247801.1 hypothetical protein -
BG586_RS09085 1786696..1787337 - 642 WP_006247800.1 hypothetical protein -
BG586_RS09090 1787369..1787764 - 396 WP_006247799.1 phage tail protein -
BG586_RS09095 1787789..1788016 - 228 Protein_1733 phage terminase large subunit family protein -
BG586_RS09100 1788166..1788540 + 375 WP_006247798.1 hypothetical protein -
BG586_RS09105 1788548..1788715 + 168 WP_006253314.1 DNA cytosine methyltransferase -
BG586_RS09110 1788796..1789119 + 324 WP_006253316.1 helix-turn-helix domain-containing protein -
BG586_RS09115 1789023..1789733 + 711 Protein_1737 tyrosine-type recombinase/integrase -
BG586_RS09125 1789834..1790469 + 636 Protein_1738 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1774276..1798482 24206
- flank IS/Tn - - 1789834..1790526 692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-75)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10278.90 Da        Isoelectric Point: 6.2121

>T67306 WP_006247804.1 NZ_CP017538:c1785599-1785330 [Mannheimia haemolytica]
MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKYRDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVR
LGSHAELFG

Download         Length: 270 bp

>T67306 NZ_CP017538:c1785599-1785330 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9913.38 Da        Isoelectric Point: 7.0082

>AT67306 WP_006247803.1 NZ_CP017538:c1785869-1785603 [Mannheimia haemolytica]
MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAKTKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEA
FHKAMLAE

Download         Length: 267 bp

>AT67306 NZ_CP017538:c1785869-1785603 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZYA9

Antitoxin

Source ID Structure
AlphaFold DB A0A547E920

References