Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1161085..1161674 Replicon chromosome
Accession NZ_CP017538
Organism Mannheimia haemolytica strain 1471

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag BG586_RS05840 Protein ID WP_006248516.1
Coordinates 1161085..1161417 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag BG586_RS05845 Protein ID WP_006248517.1
Coordinates 1161414..1161674 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG586_RS05800 1156280..1156900 - 621 WP_006248508.1 dTMP kinase -
BG586_RS05805 1156911..1157945 - 1035 WP_006248509.1 endolytic transglycosylase MltG -
BG586_RS05825 1158525..1159052 + 528 WP_006248512.1 inorganic diphosphatase -
BG586_RS05830 1159108..1159863 + 756 WP_006248513.1 M48 family metallopeptidase -
BG586_RS05835 1159927..1160958 - 1032 WP_006248514.1 tryptophan--tRNA ligase -
BG586_RS05840 1161085..1161417 - 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BG586_RS05845 1161414..1161674 - 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BG586_RS05850 1161744..1162412 - 669 WP_006248518.1 phosphoglycolate phosphatase -
BG586_RS05855 1162499..1163296 - 798 WP_006248519.1 prolipoprotein diacylglyceryl transferase -
BG586_RS05860 1163304..1164092 - 789 WP_006248520.1 sulfite exporter TauE/SafE family protein -
BG586_RS05865 1164094..1164738 - 645 WP_006248521.1 RNA pyrophosphohydrolase -
BG586_RS05870 1165350..1166495 + 1146 WP_006248526.1 FMN-dependent L-lactate dehydrogenase LldD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T67304 WP_006248516.1 NZ_CP017538:c1161417-1161085 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

>T67304 NZ_CP017538:c1161417-1161085 [Mannheimia haemolytica]
ATGAAACGTGGCGATATTTACTGGCTCGATCTTGATCCAACCTTAGGACACGAACAATCAGGCTTCCGCCCCGTTGTAAT
TGTTTCCGCAACTGCATTTAATCTAGCCAGTAAACTGCCTGTGGTTGTGCCGATTACTAACGGTGGAGCTTTTGCTGAAA
GACTGGGCTTTGCCGTTTCATTAAAAGATGCCGGCTTAAAAACAACAGGGATTATTCGATGCGATCAACCTCGCACAGTC
GATATTGCACAACGCCATGGAAAATTTATCGAAGCCTTACCCGAAAGCCTACTTGATGAAATTATGGCAAGGGTTTCAGT
GATTTTTGAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT67304 WP_006248517.1 NZ_CP017538:c1161674-1161414 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp

>AT67304 NZ_CP017538:c1161674-1161414 [Mannheimia haemolytica]
ATGCCAACAATCGCATTACGTCAGGTCGGTGGCTCGGTTGCAATGGTGATTCCGCCTGCTTTTTTAGAGGCACTCAAGCT
CTCCGTCGGTAGCAAAGTGGATTTGTCTTTAAACGACAACCAACTCGTGATTAATCCTGCCCGCCCAAAAGTGAAATACA
GCTTAGAGGAATTATTAAGACAATCCGATTTTGAGGCAGCAAGGAATGAAGAAAGCCAATCGTGGTTATCCGAGCCGGCA
ATAGGAAAAGAAATTTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZY19

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References