Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 1872750..1873289 Replicon chromosome
Accession NZ_CP017537
Organism Mannheimia haemolytica strain 1475

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZYA9
Locus tag BG608_RS09720 Protein ID WP_006247804.1
Coordinates 1873020..1873289 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A547E920
Locus tag BG608_RS09715 Protein ID WP_006247803.1
Coordinates 1872750..1873016 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG608_RS09660 1868150..1868785 - 636 Protein_1875 transposase -
BG608_RS09670 1868886..1869596 - 711 Protein_1876 tyrosine-type recombinase/integrase -
BG608_RS09675 1869500..1869823 - 324 WP_006253316.1 helix-turn-helix domain-containing protein -
BG608_RS09680 1869904..1870071 - 168 WP_006253314.1 DNA cytosine methyltransferase -
BG608_RS09685 1870079..1870453 - 375 WP_006247798.1 hypothetical protein -
BG608_RS09690 1870603..1870830 + 228 Protein_1880 phage terminase large subunit family protein -
BG608_RS09695 1870855..1871250 + 396 WP_006247799.1 phage tail protein -
BG608_RS09700 1871282..1871923 + 642 WP_006247800.1 hypothetical protein -
BG608_RS09705 1872006..1872407 + 402 WP_006247801.1 hypothetical protein -
BG608_RS09710 1872452..1872682 + 231 WP_006247802.1 DUF4035 domain-containing protein -
BG608_RS09715 1872750..1873016 + 267 WP_006247803.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BG608_RS09720 1873020..1873289 + 270 WP_006247804.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
BG608_RS09725 1873364..1873627 + 264 WP_006247805.1 hypothetical protein -
BG608_RS09730 1873687..1874397 + 711 WP_006253312.1 tape measure protein -
BG608_RS09735 1874375..1874665 + 291 Protein_1889 C40 family peptidase -
BG608_RS09740 1874655..1875374 + 720 WP_006247806.1 preprotein translocase subunit YajC -
BG608_RS09745 1875596..1876222 + 627 WP_006247807.1 tail assembly protein -
BG608_RS09750 1876271..1876882 + 612 WP_006253310.1 hypothetical protein -
BG608_RS09755 1876993..1877679 + 687 WP_006247808.1 hypothetical protein -
BG608_RS14075 1877689..1877847 + 159 WP_006247809.1 hypothetical protein -
BG608_RS09760 1877856..1878155 - 300 WP_006247810.1 XRE family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1858843..1884343 25500
- flank IS/Tn - - 1868093..1868785 692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-75)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10278.90 Da        Isoelectric Point: 6.2121

>T67297 WP_006247804.1 NZ_CP017537:1873020-1873289 [Mannheimia haemolytica]
MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKYRDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVR
LGSHAELFG

Download         Length: 270 bp

>T67297 NZ_CP017537:1873020-1873289 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9913.38 Da        Isoelectric Point: 7.0082

>AT67297 WP_006247803.1 NZ_CP017537:1872750-1873016 [Mannheimia haemolytica]
MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAKTKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEA
FHKAMLAE

Download         Length: 267 bp

>AT67297 NZ_CP017537:1872750-1873016 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZYA9

Antitoxin

Source ID Structure
AlphaFold DB A0A547E920

References