Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1451182..1451830 Replicon chromosome
Accession NZ_CP017537
Organism Mannheimia haemolytica strain 1475

Toxin (Protein)


Gene name hicA Uniprot ID A0A249A099
Locus tag BG608_RS07345 Protein ID WP_006251221.1
Coordinates 1451182..1451358 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A248ZZL6
Locus tag BG608_RS07350 Protein ID WP_006251222.1
Coordinates 1451414..1451830 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG608_RS07320 1446500..1448995 - 2496 WP_006251216.1 phage tail length tape measure family protein -
BG608_RS07330 1449540..1449998 - 459 WP_006251218.1 hypothetical protein -
BG608_RS07335 1450167..1450400 + 234 WP_020824316.1 hypothetical protein -
BG608_RS07340 1450415..1451086 - 672 WP_006251220.1 hypothetical protein -
BG608_RS07345 1451182..1451358 + 177 WP_006251221.1 type II toxin-antitoxin system HicA family toxin Toxin
BG608_RS07350 1451414..1451830 + 417 WP_006251222.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG608_RS07355 1451870..1452352 - 483 WP_006251223.1 phage tail protein -
BG608_RS07360 1452356..1452736 - 381 WP_006251224.1 hypothetical protein -
BG608_RS07365 1452733..1453101 - 369 WP_006251225.1 hypothetical protein -
BG608_RS07370 1453103..1453447 - 345 WP_006251226.1 hypothetical protein -
BG608_RS07375 1453447..1453824 - 378 WP_006251227.1 hypothetical protein -
BG608_RS07380 1453827..1454150 - 324 WP_147037499.1 hypothetical protein -
BG608_RS07385 1454162..1455151 - 990 WP_006251229.1 encapsulin -
BG608_RS07390 1455170..1455598 - 429 WP_147037500.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1434092..1488373 54281


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-64)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6640.79 Da        Isoelectric Point: 10.7708

>T67296 WP_006251221.1 NZ_CP017537:1451182-1451358 [Mannheimia haemolytica]
VKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELKTGLVEGVKKQLGLK

Download         Length: 177 bp

>T67296 NZ_CP017537:1451182-1451358 [Mannheimia haemolytica]
GTGAAGCAAAGCGAATTCCTAAGGTGGCTAAAGGCTAATGGGGTAGAGGTTGAAAATGGATCAAAGCATTTGAAACTCTA
CTACAAGGGCAAAAGAAGCCACCTCCCCAAACACCCAAGCCAAGAGTTAAAAACAGGCTTAGTGGAAGGGGTTAAAAAGC
AACTAGGCTTAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15519.91 Da        Isoelectric Point: 4.3508

>AT67296 WP_006251222.1 NZ_CP017537:1451414-1451830 [Mannheimia haemolytica]
MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLLSCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKV
LLHNTMQEQAVSKAEIARLTNIRPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG

Download         Length: 417 bp

>AT67296 NZ_CP017537:1451414-1451830 [Mannheimia haemolytica]
ATGTTTTATCCAGCGTTATTTACCCCCGCTGAAGAAGGGGGCTTTGTAGTGACATTTCCTGATTTGCCAGAAGCTATCAC
CCAAGGCGATACATTTGAAGAAGCAATGGAAATGGCAGAAGACGTGCTACTATCTTGTGTTGAAATTTATTTCGATGAAG
ATAGACGCTTTCCGCTCACTCGCCCAGTAAATACCGACGAAGTGCCTGTGTTTATGCCAGAGAGCATCTACGCAAAAGTG
CTTTTGCATAATACAATGCAAGAACAAGCGGTAAGCAAGGCAGAAATTGCCCGATTAACTAATATCCGTCCGCCAGAAGT
ACAGCGAATTTTAGCCCCTCGCCATATTACAAAAATCGACACTATTGGTCGTATTTTGGCTAGCTTAGGAAAGCCCCTAC
AACTATCCCTAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A099

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZZL6

References