Detailed information of TA system TA067295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 1132985..1133625
• Replicon: chromosome
• Accession: NZ_CP017537
• Organism: Mannheimia haemolytica strain 1475

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A378NFX7
• Locus tag: BG608_RS05785
• Protein ID: WP_006250075.1
• Coordinates: 1133443..1133625 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378NFW9
• Locus tag: BG608_RS05780
• Protein ID: WP_006250074.1
• Coordinates: 1132985..1133404 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG608_RS05755	1128498..1129304	+	807	WP_006248821.1	tryptophan synthase subunit alpha	-
BG608_RS05760	1129354..1130760	+	1407	WP_006253513.1	YdgA family protein	-
BG608_RS05765	1130827..1131867	-	1041	WP_015586960.1	IS481 family transposase	-
BG608_RS05775	1132005..1132649	-	645	WP_006253274.1	tyrosine-type recombinase/integrase	-
BG608_RS05780	1132985..1133404	-	420	WP_006250074.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BG608_RS05785	1133443..1133625	-	183	WP_006250075.1	type II toxin-antitoxin system HicA family toxin	Toxin
BG608_RS05790	1133756..1134241	-	486	WP_015484250.1	hypothetical protein	-
BG608_RS05795	1134246..1134626	-	381	WP_006248823.1	hypothetical protein	-
BG608_RS05800	1134701..1135384	-	684	WP_006248824.1	helix-turn-helix transcriptional regulator	-
BG608_RS05805	1135515..1135715	+	201	WP_006248825.1	helix-turn-helix domain-containing protein	-
BG608_RS13965	1135736..1136803	+	1068	WP_006253284.1	hypothetical protein	-
BG608_RS13970	1136813..1136971	+	159	WP_006247809.1	hypothetical protein	-
BG608_RS05820	1137031..1138071	-	1041	WP_147037492.1	IS481 family transposase	-
BG608_RS05830	1138188..1138487	-	300	WP_006247810.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1075402..1142428	67026
	-	flank	IS/Tn	-	-	1130827..1131867	1040
	-	flank	IS/Tn	-	-	1137031..1138071	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-96)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6933.03 Da
• Isoelectric Point: 10.3446

>T67295 WP_006250075.1 NZ_CP017537:c1133625-1133443 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Nucleotide

• Length: 183 bp

>T67295 NZ_CP017537:c1133625-1133443 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15938.30 Da
• Isoelectric Point: 4.6963

>AT67295 WP_006250074.1 NZ_CP017537:c1133404-1132985 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Nucleotide

• Length: 420 bp

>AT67295 NZ_CP017537:c1133404-1132985 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Toxin

• AlphaFold DB: A0A378NFX7

Antitoxin

• AlphaFold DB: A0A378NFW9