Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 1912288..1912827 Replicon chromosome
Accession NZ_CP017536
Organism Mannheimia haemolytica strain 1476

Toxin (Protein)


Gene name relE Uniprot ID A0A248ZYA9
Locus tag BG582_RS09965 Protein ID WP_006247804.1
Coordinates 1912558..1912827 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A547E920
Locus tag BG582_RS09960 Protein ID WP_006247803.1
Coordinates 1912288..1912554 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG582_RS09905 1907688..1908323 - 636 Protein_1926 transposase -
BG582_RS09915 1908424..1909134 - 711 Protein_1927 tyrosine-type recombinase/integrase -
BG582_RS09920 1909038..1909361 - 324 WP_006253316.1 helix-turn-helix domain-containing protein -
BG582_RS09925 1909442..1909609 - 168 WP_006253314.1 DNA cytosine methyltransferase -
BG582_RS09930 1909617..1909991 - 375 WP_006247798.1 hypothetical protein -
BG582_RS09935 1910141..1910368 + 228 Protein_1931 phage terminase large subunit family protein -
BG582_RS09940 1910393..1910788 + 396 WP_006247799.1 phage tail protein -
BG582_RS09945 1910820..1911461 + 642 WP_006247800.1 hypothetical protein -
BG582_RS09950 1911544..1911945 + 402 WP_006247801.1 hypothetical protein -
BG582_RS09955 1911990..1912220 + 231 WP_006247802.1 DUF4035 domain-containing protein -
BG582_RS09960 1912288..1912554 + 267 WP_006247803.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BG582_RS09965 1912558..1912827 + 270 WP_006247804.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
BG582_RS09970 1912902..1913165 + 264 WP_006247805.1 hypothetical protein -
BG582_RS09975 1913225..1913935 + 711 WP_006253312.1 tape measure protein -
BG582_RS09980 1913913..1914203 + 291 Protein_1940 C40 family peptidase -
BG582_RS09985 1914193..1914912 + 720 WP_006247806.1 preprotein translocase subunit YajC -
BG582_RS09990 1915134..1915760 + 627 WP_006247807.1 tail assembly protein -
BG582_RS09995 1915809..1916420 + 612 WP_006253310.1 hypothetical protein -
BG582_RS10000 1916531..1917217 + 687 WP_006247808.1 hypothetical protein -
BG582_RS14355 1917227..1917385 + 159 WP_006247809.1 hypothetical protein -
BG582_RS10005 1917394..1917693 - 300 WP_006247810.1 XRE family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1898371..1922455 24084
- flank IS/Tn - - 1907631..1908323 692


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-88)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-75)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10278.90 Da        Isoelectric Point: 6.2121

>T67289 WP_006247804.1 NZ_CP017536:1912558-1912827 [Mannheimia haemolytica]
MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKYRDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVR
LGSHAELFG

Download         Length: 270 bp

>T67289 NZ_CP017536:1912558-1912827 [Mannheimia haemolytica]
ATGCTACAAATTTCGCCGACAAACGCATATAAAAGAGACTTTAAAAAGATTGCAGCCGAATTAGTCGGTAGTTCGGAATA
TGTGGAAGTAATGTATTGCCTAATAAACCAATTACCATTGGCGGAAAAATATAGAGATCACCCACTACAAGGTGAATGGC
AAGGCTTTAGAGATTGCCATATTAAGCCTGATTTAGTGTTGATTTACGCTGTTGAAGATAATCTGCTCCGCCTTGTTCGT
TTAGGATCGCACGCTGAATTATTTGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9913.38 Da        Isoelectric Point: 7.0082

>AT67289 WP_006247803.1 NZ_CP017536:1912288-1912554 [Mannheimia haemolytica]
MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAKTKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEA
FHKAMLAE

Download         Length: 267 bp

>AT67289 NZ_CP017536:1912288-1912554 [Mannheimia haemolytica]
ATGGCGACTATCAATGATGCTTTCAGTTTTAGAACAAATACCGAAATAAAAAATACCGCATTTGATGTAATTAAAAACTA
TGGAATGACACCCTCTCAGGTGTTTAATATGTTTTTAACCGAGATTGCAAAAACGAAAACTATTCCGTTAAGTTTGAATT
ATCAACCCAATCTTGAAACAAAATTGGCAATGCAAGAAGCAAAATCAGGTAAAAATGAAGTTTATGCCTCACTTGAAGCA
TTTCATAAAGCAATGTTAGCGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZYA9

Antitoxin

Source ID Structure
AlphaFold DB A0A547E920

References