Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 1129219..1129859 Replicon chromosome
Accession NZ_CP017536
Organism Mannheimia haemolytica strain 1476

Toxin (Protein)


Gene name hicA Uniprot ID A0A378NFX7
Locus tag BG582_RS05760 Protein ID WP_006250075.1
Coordinates 1129677..1129859 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A378NFW9
Locus tag BG582_RS05755 Protein ID WP_006250074.1
Coordinates 1129219..1129638 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG582_RS05730 1124766..1125572 + 807 WP_006248821.1 tryptophan synthase subunit alpha -
BG582_RS05735 1125622..1127028 + 1407 WP_006253513.1 YdgA family protein -
BG582_RS05740 1127095..1128101 - 1007 Protein_1118 IS481 family transposase -
BG582_RS05750 1128239..1128883 - 645 WP_006253274.1 tyrosine-type recombinase/integrase -
BG582_RS05755 1129219..1129638 - 420 WP_006250074.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BG582_RS05760 1129677..1129859 - 183 WP_006250075.1 type II toxin-antitoxin system HicA family toxin Toxin
BG582_RS05765 1129991..1130476 - 486 WP_015484250.1 hypothetical protein -
BG582_RS05770 1130481..1130861 - 381 WP_006248823.1 hypothetical protein -
BG582_RS05775 1130936..1131619 - 684 WP_006248824.1 helix-turn-helix transcriptional regulator -
BG582_RS05780 1131750..1131950 + 201 WP_006248825.1 helix-turn-helix domain-containing protein -
BG582_RS14225 1131971..1133038 + 1068 WP_006253284.1 hypothetical protein -
BG582_RS14230 1133048..1133206 + 159 WP_006247809.1 hypothetical protein -
BG582_RS05795 1133266..1134306 - 1041 WP_015587043.1 IS481 family transposase -
BG582_RS05805 1134423..1134722 - 300 WP_006247810.1 XRE family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1133266..1134306 1040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-58)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-96)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.03 Da        Isoelectric Point: 10.3446

>T67287 WP_006250075.1 NZ_CP017536:c1129859-1129677 [Mannheimia haemolytica]
MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKEIGTGLVNKIKKDLDLK

Download         Length: 183 bp

>T67287 NZ_CP017536:c1129859-1129677 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15938.30 Da        Isoelectric Point: 4.6963

>AT67287 WP_006250074.1 NZ_CP017536:c1129638-1129219 [Mannheimia haemolytica]
MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGLATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMK
VFLLNEMIKQGVRKAEMARRLDVHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH

Download         Length: 420 bp

>AT67287 NZ_CP017536:c1129638-1129219 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378NFX7

Antitoxin

Source ID Structure
AlphaFold DB A0A378NFW9

References